High temperatures promote antibiotic resistance genes conjugative transfer under residual chlorine: Mechanisms and risks

IF 12.2 1区 环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL
Wenya Zhao , Binghuang Zhang , Shikan Zheng , Wanli Yan , Xin Yu , Chengsong Ye
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Abstract

The impact of residual chlorine on the dissemination of antibiotic resistance during the distribution and storage of water has become a critical concern. However, the influence of rising temperatures attributed to global warming on this process remains ambiguous, warranting further investigation. This study investigated the effects of different temperatures (17, 27, 37, and 42°C) on the conjugative transfer of antibiotic resistance genes (ARGs) under residual chlorine (0, 0.1, 0.3, and 0.5 mg/L). The results indicated that high temperatures significantly increased the conjugative transfer frequency of ARGs in intra-species under residual chlorine. Compared to 17°C, the transfer frequencies at 27°C, 37°C, and 42°C increased by 1.07–2.43, 1.20–4.80, and 1.24–2.82 times, respectively. The promoting effect of high temperatures was mainly due to the generation of reactive oxygen species, the triggered SOS response, and the formation of pilus channels. Transcriptomic analysis demonstrated that higher temperature stimulates the electron transport chain, thereby enhancing ATP production and facilitating the processes of conjugative, as confirmed by inhibitor validation. Additionally, rising temperatures similarly promoted the frequency of conjugative transfer in inter-species and communities under residual chlorine. These further highlighted the risk of antibiotic resistance spread in extreme and prolonged high-temperature events. The increased risk of antibiotic resistance in the process of drinking water transmission under the background of climate warming is emphasized.

Abstract Image

高温促进抗生素耐药基因在余氯条件下的共轭转移:机制与风险
在水的分配和储存过程中,余氯对抗生素耐药性传播的影响已成为一个重要问题。然而,全球变暖导致的气温升高对这一过程的影响仍不明确,值得进一步研究。本研究调查了在余氯(0、0.1、0.3 和 0.5 mg/L)条件下,不同温度(17、27、37 和 42˚C)对抗生素抗性基因(ARGs)共轭转移的影响。结果表明,在余氯条件下,高温明显增加了种内 ARGs 的共轭传递频率。与 17˚C 相比,27˚C、37˚C 和 42˚C 下的转移频率分别增加了 1.07-2.43、1.20-4.80 和 1.24-2.82 倍。高温的促进作用主要是由于活性氧的产生、SOS 反应的触发和柔毛通道的形成。转录组分析表明,较高的温度刺激了电子传递链,从而提高了 ATP 的产生并促进了共轭过程,抑制剂验证也证实了这一点。此外,在余氯条件下,温度升高同样会促进种间和群落间的共轭转移频率。这进一步凸显了抗生素耐药性在极端和长期高温事件中传播的风险。强调了在气候变暖的背景下,饮用水传播过程中抗生素耐药性风险的增加。
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来源期刊
Journal of Hazardous Materials
Journal of Hazardous Materials 工程技术-工程:环境
CiteScore
25.40
自引率
5.90%
发文量
3059
审稿时长
58 days
期刊介绍: The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.
文献相关原料
公司名称 产品信息 采购帮参考价格
麦克林 Streptomycin
麦克林 Tetracycline
麦克林 Chloramphenicol
麦克林 Ampicillin
麦克林 Kanamycin
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