Antifungal efficacy and biofumigation potential of hydrophobic deep eutectic solvents: Postharvest treatment against Monilinia fructicola and Botrytis Cinerea.

Abstract

Hydrophobic Deep Eutectic Solvents (HDES), as a subclass of Natural Deep Eutectic Solvents (NADES), present a green-chemistry alternative to toxic chemicals. As HDES are based on terpenoids, these solvents could potentially be effective antifungal agents against phytopathogens Monilinia fructicola and Botrytis cinerea that frequently cause diseases in sweet cherry fruit. To contribute to the disease prevention and management goals, as a part of this study, 30 different HDES were tested in the vapor phase, at identical concentrations of 25%, 50%, and 100%. In vitro experiments were conducted on Potato Dextrose Agar medium (PDA), while in planta experiments were carried out in hermetically sealed containers with inoculated sweet cherry fruits. All tested HDES demonstrated efficacy in suppressing the growth of M. fructicola colonies (66 - 100%) and B. cinerea colonies (37 - 100%). According to the Area Under the Disease Progress Curve (AUDPC), all HDES exhibited high efficacy in preventing disease occurrence in cherry fruits by the tested phytopathogens. This research provides the first insights into the antifungal potential of HDES in the vapor phase, with promising applications as biofumigants that minimize harmful impacts on the food - human - environment complex.