CRISPR-Cas-based colorimetric strategies for nucleic acids detection

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2024-11-20 DOI:10.1016/j.trac.2024.118058

Yujin Yang , Xingyu Li , Xiaobo Wang, Zongji Wang, Shaohua Gong

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引用次数: 0

Abstract

Accurate and facile detection of nucleic acids is significant for diagnosis and prognosis of diseases. Compared with traditional methods, CRISPR-based diagnosis shows high sensitivity and specificity, hopefully becoming the next-generation molecular diagnosis approach. Various signal output modes have been integrated with CRISPR-Cas to achieve ultrasensitive detection of nucleic acid biomarkers. Among these signal modes, colorimetric signal attracts great attention due to its simplicity, which will contribute to the point-of-care testing of nucleic acid biomarkers. Therefore, different CRISPR-based colorimetric strategies have been designed, aiming to realize facile and ultrasensitive nucleic acid biomarkers detection. In this review, the working mechanism of four CRISPR-Cas systems is first introduced, followed by summarizing the research progress of CRISPR-Cas-based colorimetric strategies, including UV/blue light-based colorimetry, lateral flow strip-based colorimetry, AuNPs-based colorimetry in solution and enzyme-based colorimetry. Furthermore, the challenges and perspectives in this field are discussed to promote the development of CRISPR-Cas-based colorimetry in practical application.

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基于 CRISPR-Cas 的核酸比色检测策略

准确、便捷地检测核酸对疾病的诊断和预后具有重要意义。与传统方法相比，基于CRISPR的诊断具有高灵敏度和高特异性，有望成为下一代分子诊断方法。为了实现对核酸生物标志物的超灵敏检测，CRISPR-Cas 集成了多种信号输出模式。在这些信号模式中，比色信号因其简便性而备受关注，这将有助于核酸生物标志物的床旁检测。因此，人们设计了不同的基于CRISPR的比色策略，旨在实现简便、超灵敏的核酸生物标志物检测。本综述首先介绍了四种CRISPR-Cas系统的工作机制，然后总结了基于CRISPR-Cas的比色策略的研究进展，包括基于紫外/蓝光的比色法、基于侧流条带的比色法、基于溶液中AuNPs的比色法和基于酶的比色法。此外，还讨论了该领域的挑战和前景，以促进基于 CRISPR-Cas 的比色法在实际应用中的发展。

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.