Assessment of bisphenol accumulation from disposable devices used sequentially in IVF routine procedures

IF 3.7 2区医学 Q1 OBSTETRICS & GYNECOLOGY

Reproductive biomedicine online Pub Date : 2024-08-29 DOI:10.1016/j.rbmo.2024.104431

Lucie Delaroche , Lucile Besnard , Nino Guy Cassuto , Sébastien Bristeau , Anne Togola

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引用次数: 0

Abstract

Research question

Are bisphenols released from disposable devices used in assisted reproductive technology (ART) procedures, and do they accumulate when several disposable devices are used sequentially under routine conditions?

Design

A comprehensive assessment of 19 individual disposable devices (31 assessments) and nine combinations of disposable devices replicating the main steps in an ART procedure was undertaken. The extraction of bisphenols followed routine-use conditions (temperature and duration). The concentrations of 10 bisphenols were determined using online solid-phase extraction/liquid chromatography/mass spectrometry methodology.

Results

Bisphenol S (BPS) was quantified consistently from 100-mm culture dishes (32 ± 20 pg) and from high security sperm straws (3 ± 1 pg). Also, BPS and bisphenol A (BPA) were quantified consistently from spermicide-free condoms (95 ± 78 and 83 ± 49 pg, respectively). No other bisphenols were detected in disposable devices when tested individually. When disposable devices were used in combination, both BPA and BPS were detected consistently in combinations of 13 disposable devices mimicking sperm collection in a condom and its preparation (46 ± 16 and 43 ± 32 pg, respectively). BPS was quantified consistently in combinations of 14 disposable devices mimicking sperm collection, its preparation and freezing (10 ± 4 pg), and in combinations of 17 disposable devices mimicking oocyte retrieval (37 ± 22 pg).

Conclusions

BPA and BPS are released in small quantities from some disposable devices used in routine conditions during ART procedures, but do not appear to accumulate when these disposable devices are used in combination.

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评估试管婴儿常规程序中连续使用的一次性设备中双酚的累积情况

研究问题辅助生殖技术（ART）程序中使用的一次性设备是否会释放双酚，以及在常规条件下连续使用多个一次性设备时是否会累积双酚？双酚的提取遵循常规使用条件（温度和持续时间）。采用在线固相萃取/液相色谱/质谱方法测定了 10 种双酚的浓度。结果从 100 毫米培养皿（32 ± 20 pg）和高安全性精子吸管（3 ± 1 pg）中一致定量了双酚 S（BPS）。此外，从不含杀精剂的避孕套中也能稳定地定量检测到双酚 S 和双酚 A（BPA）（分别为 95 ± 78 和 83 ± 49 pg）。单独检测一次性装置时，没有检测到其他双酚。当组合使用一次性装置时，在 13 个一次性装置的组合中检测到双酚 A 和双酚 BPS（分别为 46 ± 16 pg 和 43 ± 32 pg）。在模拟精子收集、精子制备和冷冻的 14 种一次性装置组合中（10 ± 4 pg），以及模拟卵母细胞提取的 17 种一次性装置组合中（37 ± 22 pg），双酚 A 和双酚 B 的定量一致。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproductive biomedicine online 医学-妇产科学

CiteScore

7.20

自引率

7.50%

发文量

391

审稿时长

50 days

期刊介绍： Reproductive BioMedicine Online covers the formation, growth and differentiation of the human embryo. It is intended to bring to public attention new research on biological and clinical research on human reproduction and the human embryo including relevant studies on animals. It is published by a group of scientists and clinicians working in these fields of study. Its audience comprises researchers, clinicians, practitioners, academics and patients. Context: The period of human embryonic growth covered is between the formation of the primordial germ cells in the fetus until mid-pregnancy. High quality research on lower animals is included if it helps to clarify the human situation. Studies progressing to birth and later are published if they have a direct bearing on events in the earlier stages of pregnancy.