LcCCoAOMT8 from Liriodendron chinense positively regulates lignin synthesis and promotes secondary cell wall thickening in tobacco (Nicotiana tabacum)

IF 5.6 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products Pub Date : 2024-11-26 DOI:10.1016/j.indcrop.2024.120137

Manli Bu, Kaifei Xing, Wei Li, Lichun Yang, Yaxian Zong, Junpeng Wu, Huogen Li

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引用次数: 0

Abstract

Caffeoyl coenzyme A-O-methyltransferase (CCoAOMT) is engaged in plant secondary metabolism and stress response, influencing the synthesis of lignin. Liriodendron chinense is an excellent timber species, while the potential mechanisms of wood formation in L. chinense remains unclear. Here, we identified the CCoAOMT gene family within the L. chinense genome and analyzed the role of LcCCoAOMT8 in regulating lignin biosynthesis. In total, eight LcCCoAOMT genes were categorized into four distinct groups according to their phylogenetic characteristics. RNA-seq data analysis revealed that LcCCoAOMT1, LcCCoAOMT3, LcCCoAOMT5, and LcCCoAOMT8 were highly expressed in the developing xylem of the stem (DXS). Further RT-qPCR results confirmed that LcCCoAOMT8 was most highly expressed in the stem. Moreover, overexpression of LcCCoAOMT8 in transgenic tobacco plants increased CCoAOMT activity and lignin content, promoted xylem development, and enhanced secondary cell wall (SCW) thickening. In summary, our results suggest that LcCCoAOMT8 positively regulates lignin synthesis in stem and promotes SCW thickening.

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来自 Liriodendron chinense 的 LcCCoAOMT8 可正向调节烟草（Nicotiana tabacum）木质素合成并促进次生细胞壁增厚

咖啡酰辅酶 A-O-甲基转移酶（CCoAOMT）参与植物次生代谢和胁迫响应，影响木质素的合成。鹅掌楸是优良的用材树种，但鹅掌楸木材形成的潜在机制仍不清楚。在此，我们确定了鹅掌楸基因组中的 CCoAOMT 基因家族，并分析了 LcCCoAOMT8 在调控木质素生物合成中的作用。根据系统发育特征，共将 8 个 LcCCoAOMT 基因分为 4 个不同的组。RNA-seq 数据分析显示，LcCCoAOMT1、LcCCoAOMT3、LcCCoAOMT5 和 LcCCoAOMT8 在茎发育木质部（DXS）中高表达。进一步的 RT-qPCR 结果证实，LcCCoAOMT8 在茎中的表达量最高。此外，在转基因烟草植株中过表达 LcCCoAOMT8 能提高 CCoAOMT 活性和木质素含量，促进木质部发育，增强次生细胞壁（SCW）增厚。总之，我们的研究结果表明，LcCCoAOMT8 能正向调节茎中木质素的合成并促进 SCW 增厚。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Industrial Crops and Products 农林科学-农业工程

CiteScore

9.50

自引率

8.50%

发文量

1518

审稿时长

43 days

期刊介绍： Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.