Assessment of waste anesthetic gas concentrations in the operating theaters and DNA damage in exposed female nurse anesthetists

IF 2.3 Q2 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Clinical Epidemiology and Global Health Pub Date : 2024-11-01 DOI:10.1016/j.cegh.2024.101841

Thitiworn Choosong , Jatuporn Pakpirom

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引用次数: 0

Abstract

Background

Personnel that work in operation theaters are at risk of exposure to waste anesthetic gases (WAGs) that can cause deoxyribonucleic acid (DNA) damage. To assess the potential impacts of single strand break levels of nurse anesthetists, WAGs levels, and other factors were investigated.

Methods

Non-smoker nurse anesthetists (exposure group) and registered nurses (control group) that have never worked in operating theaters (OT) were matched by age. The WAG concentrations in OT were measured via a direct reading instrument. A blood sample of 2 mL from each volunteer nurse was used to determine the single strand break of DNA damage by comet assay; between July 2014 and January 2016.

Results

In total 77 nurses participated in this study. Even if the average 8-h TWA of the WAG concentrations were low, N₂O peaked at 951 ppm and sevoflurane peaked at 88.3 ppm during the induction phrase of pediatric patients. Multivariate analysis showed that ln (% of DNA) in the tail and ln (tail moment) were positively, significantly associated with N₂O and cancer in family history, while ln (tail length) was not. However, dietary consumption of fish, meat and cruciferous vegetable had influence on DNA damage and shift work should not be neglected.

Conclusion

Nurse anesthetists were potentially exposed to very high WAG concentrations; especially in pediatric cases. There were multi-factors associated with DNA damage score. To decrease the DNA damage score, lifestyle and dietary consumption should be of more concern.

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评估手术室中麻醉废气的浓度和接触废气的麻醉女护士的 DNA 损伤情况

背景在手术室工作的人员有接触麻醉废气（WAGs）的风险，WAGs 可导致脱氧核糖核酸（DNA）损伤。为了评估单链断裂水平对麻醉护士的潜在影响，我们对 WAGs 水平和其他因素进行了调查。方法将从未在手术室（OT）工作过的非吸烟麻醉护士（暴露组）和注册护士（对照组）按年龄进行配对。通过直读仪器测量手术室中的 WAG 浓度。在 2014 年 7 月至 2016 年 1 月期间，每位志愿护士抽取 2 mL 血液样本，通过彗星测定法确定 DNA 的单链断裂损伤情况。即使 WAG 的平均 8 小时 TWA 浓度较低，但在儿科患者的诱导过程中，一氧化二氮的峰值为 951 ppm，七氟醚的峰值为 88.3 ppm。多变量分析表明，尾部的ln（DNA%）和ln（尾矩）与N2O和癌症家族史呈显著正相关，而ln（尾长）则不相关。然而，饮食中的鱼、肉和十字花科蔬菜对 DNA 损伤有影响，轮班工作也不容忽视。DNA损伤评分与多种因素有关。要降低 DNA 损伤评分，生活方式和饮食消费应受到更多关注。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical Epidemiology and Global Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-

CiteScore

4.60

自引率

7.70%

发文量

218

审稿时长

66 days

期刊介绍： Clinical Epidemiology and Global Health (CEGH) is a multidisciplinary journal and it is published four times (March, June, September, December) a year. The mandate of CEGH is to promote articles on clinical epidemiology with focus on developing countries in the context of global health. We also accept articles from other countries. It publishes original research work across all disciplines of medicine and allied sciences, related to clinical epidemiology and global health. The journal publishes Original articles, Review articles, Evidence Summaries, Letters to the Editor. All articles published in CEGH are peer-reviewed and published online for immediate access and citation.