Syk/Src/NF-κB axis is essentially targeted in anti-inflammatory and anti-gastritis effects of Bletilla striata ethanol extract

IF 4.8 2区医学 Q1 CHEMISTRY, MEDICINAL

Journal of ethnopharmacology Pub Date : 2024-11-22 DOI:10.1016/j.jep.2024.119155

Ji Yeon Hwang , Mi-Yeon Kim , Jae Youl Cho

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引用次数: 0

Abstract

Ethnopharmacological relevance

Traditional herbal medicine books “Shin Rhong Bon Cho Kyung” and “Hyang Yak Jip Sung Bang” mentioned that Bletilla striata (Thunb.) Rchb.f. (Orchidaceae) was often used as a medicinal plant and is used in oriental medicine to treat wounds, inflammatory symptoms, and ulcers in stomach, lung, and skin. However, systematic studies on its value as a promising anti-inflammatory remedy were not fully elucidated yet.

Aim of the study: The eventual goal of this paper was to explore anti-inflammatory and anti-gastritis effects of Bletilla striata and its inhibitory mechanism with an ethanol extract of this plant (Bs-EE).

Materials and methods

In vitro study includes nitric oxide (NO) inhibitory test by was Griess assay, cell viability check by MTT assay, mRNA level analysis of inflammatory genes by PCR and RT-PCR, and protein level analysis by Western blotting and CESTA. In vivo analysis was done with a mouse gastritis model triggered by HCl/EtOH. Phytochemical finger printing result was observed by GC/MS-MS.

Results

Our in vitro trials showed that Bs-EE dose-dependently reduced NO production in lipopolysaccharide-, Poly(I:C)-, and Pam3CSK-treated RAW264.7 cells without causing cytotoxicity, as shown by an MTT assay. The levels of inflammation-related genes (iNOS, IL-6, IL-1β) showed meaningful reductions in RT-PCR and real-time PCR. The NF-κB activity enhanced in MyD88-overexpressing HEK293T cells was strongly reduced by Bs-EE. Western blotting results indicated that the Bs-EE suppressed the phosphorylation of IκBα, IKKα/β, AKT, p65, p50, Syk, and Src, which produced anti-inflammatory effects. Both Syk and Src were found to be direct targets of Bs-EE. This extract attenuated the inflammatory effect in a murine acute gastritis model induced by HCl/EtOH.

Conclusions

These findings suggest that an ethanol extract of Bletilla striata could be developed as a promising natural anti-inflammatory drug or health functional food with NF-κB pathway inhibitory activity.

Abstract Image

查看原文本刊更多论文

芨芨草乙醇提取物的抗炎和抗胃炎作用主要针对 Syk/Src/NF-κB 轴。

民族药理学相关性：传统草药书籍 "Shin Rhong Bon Cho Kyung "和 "Hyang Yak Jip Sung Bang "提到，Bletilla striata (Thunb.) Rchb.f.（兰科）经常被用作药用植物，在东方医学中用于治疗伤口、炎症症状以及胃、肺和皮肤溃疡。然而，关于其作为一种有前途的抗炎药物的价值的系统研究尚未完全阐明：研究目的：本文的最终目的是探索条叶紫苏的抗炎和抗胃炎作用及其乙醇提取物（Bs-EE）的抑制机制：体外研究包括一氧化氮（NO）抑制试验（Griess 试验）、细胞活力检测（MTT 试验）、炎症基因 mRNA 水平分析（PCR 和 RT-PCR）以及蛋白质水平分析（Western 印迹和 CESTA）。使用 HCl/EtOH 引发的小鼠胃炎模型进行了体内分析。通过 GC/MS-MS 观察了植物化学指纹图谱结果：我们的体外试验表明，Bs-EE 能剂量依赖性地减少脂多糖、Poly(I:C)和 Pam3CSK 处理的 RAW264.7 细胞中 NO 的产生，而不会引起细胞毒性。在 RT-PCR 和实时 PCR 中，炎症相关基因（iNOS、IL-6、IL-1β）的水平出现了明显的下降。Bs-EE 强力降低了过表达 MyD88 的 HEK293T 细胞中增强的 NF-κB 活性。Western blotting结果表明，Bs-EE能抑制IκBα、IKKα/β、AKT、p65、p50、Syk和Src的磷酸化，从而产生抗炎作用。研究发现，Syk 和 Src 都是 Bs-EE 的直接靶标。在 HCl/EtOH 诱导的小鼠急性胃炎模型中，该提取物可减轻炎症效应：这些研究结果表明，紫苏的乙醇提取物可以开发成一种具有 NF-κB 通路抑制活性的天然抗炎药物或保健功能食品。

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来源期刊

Journal of ethnopharmacology 医学-全科医学与补充医学

CiteScore

10.30

自引率

5.60%

发文量

967

审稿时长

77 days

期刊介绍： The Journal of Ethnopharmacology is dedicated to the exchange of information and understandings about people''s use of plants, fungi, animals, microorganisms and minerals and their biological and pharmacological effects based on the principles established through international conventions. Early people confronted with illness and disease, discovered a wealth of useful therapeutic agents in the plant and animal kingdoms. The empirical knowledge of these medicinal substances and their toxic potential was passed on by oral tradition and sometimes recorded in herbals and other texts on materia medica. Many valuable drugs of today (e.g., atropine, ephedrine, tubocurarine, digoxin, reserpine) came into use through the study of indigenous remedies. Chemists continue to use plant-derived drugs (e.g., morphine, taxol, physostigmine, quinidine, emetine) as prototypes in their attempts to develop more effective and less toxic medicinals.