OsPUKI, a PfkB protein, regulates seed germination in rice by influencing ABA synthesis

IF 2.6 3区生物学 Q2 GENETICS & HEREDITY

Gene Pub Date : 2024-11-22 DOI:10.1016/j.gene.2024.149118

Yi Bao , Zongfei Zhang , Ni Peng, Ziting Qiu, Xin Yan, Jiexiu Ouyang, Shaobo Li, Xin Wang

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引用次数: 0

Abstract

Rice seed germination is a crucial phase in rice growth and development, but its molecular mechanism has not been fully elucidated. In this study, we investigated the function of rice pfkB family gene OsPUKI in seed germination. Compared with WT (ZH11), ospuki mutants showed delayed seed germination and shorter shoot length. QRT-PCR results showed that OsPUKI was highly expressed in leaves and developing seeds of 21-day after pollination, and was highly expressed at the early stage of seed germination. GC-MS analysis demonstrated that content of abscisic acid (ABA) in ospuki-3 was higher than that in WT. QRT-PCR analysis revealed that ospuki mutants had higher transcription levels of ABA synthesis-related genes OsNCED2, OsNCED3, OsNCED4, and OsZEP1. Furthermore, it was shown that ospuki mutants were more sensitive to fluridone (Flu) during seed germination and seedling growth than WT according to exogenous Flu treatment experiments. In short, our findings suggest that OsPUKI may positively regulates rice seed germination by influencing ABA synthesis.

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OsPUKI是一种PfkB蛋白，它通过影响ABA的合成来调节水稻种子的萌发。

水稻种子萌发是水稻生长发育的关键阶段，但其分子机制尚未完全阐明。本研究探讨了水稻 pfkB 家族基因 OsPUKI 在种子萌发中的功能。与WT（ZH11）相比，ospuki突变体表现出种子萌发延迟和芽长度缩短。QRT-PCR结果显示，OsPUKI在授粉后21天的叶片和发育中的种子中高表达，并在种子萌发早期高表达。气相色谱-质谱（GC-MS）分析表明，ospuki-3 的脱落酸（ABA）含量高于 WT。QRT-PCR 分析表明，ospuki 突变体中 ABA 合成相关基因 OsNCED2、OsNCED3、OsNCED4 和 OsZEP1 的转录水平较高。此外，外源Flu处理实验表明，ospuki突变体在种子萌发和幼苗生长过程中对氟啶酮（Flu）比WT更敏感。总之，我们的研究结果表明，OsPUKI可能通过影响ABA的合成来积极调控水稻种子的萌发。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene 生物-遗传学

CiteScore

6.10

自引率

2.90%

发文量

718

审稿时长

42 days

期刊介绍： Gene publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses.