Characterization of a Novel Tn7-like Transposon Carrying blaDHA-1 in Providencia stuartii MF1 Isolated from Swine Wastewater.

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2024-11-23 DOI:10.1007/s00284-024-03952-x

Shijie Ou, Xinyi Tian, Zhen Zhang, Lingjia Zhu, Rong Wang, Guangxiang Cao, Jiafang Fu, Peipei Zhang

引用次数: 0

Abstract

Providencia stuartii is an emerging pathogen that causes nosocomial infections. In this study, a multidrug-resistant strain P. stuartii MF1 was isolated from swine wastewater. Comprehensive analysis of whole genome sequencing revealed that dozens of antibiotic resistance genes were found in MF1. A novel transposon Tn6450M which has high sequence identity to Tn6450 and the plasmid-borne Tn6765 from Proteus mirabilis was identified in the genome of MF1. Tn6450M was determined to be stably inserted into a new attTn7 site in the P. stuartii MF1 genome and contains the third-generation cephalosporins resistance-associated genes blaDHA-1. Intergeneric transmission of Tn6450 variants poses risks for the spread of antibiotic resistance genes.

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从猪废水中分离出的携带 blaDHA-1 的 Providencia stuartii MF1 的新型 Tn7 类转座子的特征。

普罗维登菌（Providencia stuartii）是一种新出现的病原体，可引起医院内感染。本研究从猪废水中分离出一株具有多重耐药性的 P. stuartii MF1。全基因组测序的综合分析表明，在 MF1 中发现了数十个抗生素耐药基因。在 MF1 的基因组中发现了一个新的转座子 Tn6450M，该转座子与 Tn6450 和奇异变形杆菌质粒上的 Tn6765 有很高的序列同一性。经测定，Tn6450M 被稳定地插入到 P. stuartii MF1 基因组中一个新的 attTn7 位点，并含有第三代头孢菌素耐药性相关基因 blaDHA-1。Tn6450变体的跨代传播为抗生素耐药基因的传播带来了风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.