Limosilactobacillus reuteri B1/1 modulated the intestinal immune response in preventing Salmonella Enteritidis PT4 infection in a chicken ileal explant model.

IF 1.8 3区农林科学 Q2 VETERINARY SCIENCES

Veterinary Research Communications Pub Date : 2024-11-23 DOI:10.1007/s11259-024-10609-4

Viera Karaffová, Zuzana Kiššová, Csilla Tóthová, Patrik Tráj, Máté Mackei, Gábor Mátis

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引用次数: 0

Abstract

In this study, we observed the effect of the newly isolated probiotic strain Limosilactobacillus reuteri B1/1 on the relative gene expression of selected cytokines (interleukin-15, transforming growth factor-β4), tight junction proteins (E-cadherin, occludin), biomarker active intestinal stem cells - LGR5 (leucine-rich repeat containing G protein-coupled receptor), markers of mucosal intestinal immunity (mucin-2, immunoglobulin A), as well as the creation of a new biomarker of inflammation in the intestine - calprotectin on an ex vivo model of chicken ileal explant in the prevention of Salmonella Enteritidis PT4 infection. The ability of L. reuteri B1/1 to effectively modulate the mucosal immune response under pretreatment conditions in S. Enteritidis PT4 infection in a chicken ileal explant model was confirmed. In addition, our obtained results point to the fact that the new chicken ileum explant model could be a suitable model to investigate or test the influence of natural substances such as probiotic bacteria in the interaction with the intestine as well as pathogenic microorganisms. In addition, the results of our study may contribute to a deeper understanding of the action of newly isolated probiotic bacteria at the intestinal level using ex vivo models such as chicken ileum explant, which are able to mimic in vivo conditions sufficiently.

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在鸡回肠外植体模型中，Limosilactobacillus reuteri B1/1 可调节肠道免疫反应，预防肠炎沙门氏菌 PT4 感染。

在这项研究中，我们观察了新分离的益生菌株Limosilactobacillus reuteri B1/1对某些细胞因子（白细胞介素-15、转化生长因子-β4）、紧密连接蛋白（E-cadherin、occludin）、活跃的肠干细胞生物标志物--LGR5（富含亮氨酸重复的G蛋白偶联受体）的相对基因表达的影响、肠粘膜免疫标志物（粘蛋白-2、免疫球蛋白 A），以及在预防肠炎沙门氏菌 PT4 感染的鸡回肠外植体体外模型上创建了一种新的肠道炎症生物标志物--钙保护蛋白。在鸡回肠外植体模型中，证实了在肠炎沙门氏菌 PT4 感染的预处理条件下，L. reuteri B1/1 能够有效调节粘膜免疫反应。此外，我们的研究结果表明，新型鸡回肠外植体模型可作为研究或测试益生菌等天然物质与肠道及病原微生物相互作用影响的合适模型。此外，我们的研究结果可能有助于利用鸡回肠外植体等体内外模型，更深入地了解新分离的益生菌在肠道水平的作用，因为这种模型能够充分模拟体内条件。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary Research Communications 农林科学-兽医学

CiteScore

2.50

自引率

0.00%

发文量

173

审稿时长

3 months

期刊介绍： Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial. The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.