Gold-grafted melamine sponge as surface-enhanced Raman spectroscopy substrate for enzyme-linked immunoassay of mycotoxins in cereal samples

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2024-11-25 DOI:10.1007/s00604-024-06831-1

Xianzhi Huang, Ling Xia, Gongke Li

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Abstract

Gold-grafted melamine sponge (AuSp) was fabricated and applied as surface-enhanced Raman spectroscopy (SERS) substrate for enzyme-linked immunoassay of 2 typical mycotoxins, zearalenone (ZEN) and T-2 toxin. The gold nanoparticles were in situ grown on the sponge, and the product showed good effect in enhancing Raman signal and good stability. COMSOL and Gaussian simulations were used to analyze the enhancing effect. Some of the experimental conditions that may affect the determination results were studied in order to meet the maximum response to mycotoxins. At optimized conditions, the limits of detection of the method were 1 μg·kg⁻¹ and 0.05 μg·kg⁻¹ for ZEN and T-2 toxin, with linear ranges of 5–100 μg·kg⁻¹ and 0.1–20 μg·kg⁻¹, respectively, and relative standard deviations lower than 7.0%. The established method was used to determine ZEN and T-2 toxin in six kinds of cereal samples, the recoveries were 85–120%, and relative errors were less than 13.0%. These results indicated the accuracy of the established method. After 1-year storage, signal strength of AuSp for mycotoxins determination decreased by less than 10%, showing stability over a long time. Our work provided a new method for SERS determination of mycotoxins, which reduces the complexity of substrate preparation and the sample consumption compared with traditional methods, and thus has potential for on-line SERS determination.

Graphical Abstract

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作为表面增强拉曼光谱底物的金接枝三聚氰胺海绵用于谷物样品中霉菌毒素的酶联免疫测定

制备了金接枝三聚氰胺海绵（AuSp），并将其用作表面增强拉曼光谱（SERS）底物，用于酶联免疫检测两种典型的霉菌毒素玉米赤霉烯酮（ZEN）和T-2毒素。金纳米粒子原位生长在海绵上，该产品在增强拉曼信号方面表现出良好的效果和稳定性。利用 COMSOL 和高斯模拟分析了增强效果。研究了一些可能影响测定结果的实验条件，以满足对霉菌毒素的最大响应。在优化条件下，ZEN和T-2毒素的检出限分别为1 μg-kg-1和0.05 μg-kg-1，线性范围分别为5-100 μg-kg-1和0.1-20 μg-kg-1，相对标准偏差小于7.0%。用该方法测定了6种谷物样品中的ZEN和T-2毒素，回收率为85%～120%，相对误差小于13.0%。这些结果表明所建立的方法是准确的。贮藏一年后，用于测定霉菌毒素的 AuSp 信号强度下降不到 10%，显示了其长期稳定性。我们的工作为 SERS 法测定霉菌毒素提供了一种新方法，与传统方法相比，该方法降低了底物制备的复杂性，减少了样品消耗量，因此具有在线 SERS 测定的潜力。图文摘要

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.