New Insights into the Effects of Polystyrene Microplastics on Freshwater Fish, Labeo rohita: Assessment on Histopathology, Mineral Composition, Bioaccumulation and Antioxidant Activity

IF 3.8 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Water, Air, & Soil Pollution Pub Date : 2024-11-25 DOI:10.1007/s11270-024-07643-y

Eram Rashid, Syed Makhdoom Hussain, Shafaqat Ali, Pallab K. Sarker, Shabab Nasir, Khalid Mashay Al-Anazi, Nadia Nazish

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Abstract

Microplastics (MPs) have become a pressing concern due to their pervasive presence in the environment and bioaccumulation in fish organs, potentially threatening ecosystem health. This study examined the impact of polystyrene (PS) MPs on Labeo rohita fingerlings (7.15 ± 0.02 g/fish) by exploring histopathological changes in intestine, altered mineral composition in muscles, bioaccumulation and modulated antioxidant enzyme activity. This study utilized six test diets comprising sunflower meal with varying polystyrene microplastic (PS-MP) levels. The control diet (I) contained 0% PS-MPs, while diets II-VI had 0.5%, 1%, 1.5%, 2%, and 2.5% PS-MPs, respectively, over a 90-day feeding trial. The fish were fed their respective test diets twice daily with a feeding rate of 5% wet body weight per day. The findings elucidated that the antioxidant enzyme activity and bioaccumulation were substantially altered by increased PS-MPs exposure. Moreover, histopathology examination revealed intestinal structural abnormalities, which worsened with escalating PS-MPs concentrations. Notably, test diet VI (2.5% PS-MPs) showed lower mineral content (P < 0.05) in fingerlings muscles when compared with control. In conclusion, results indicate that PS-MPs may negatively impact the L. rohita fingerlings, affecting histopathology of intestine, mineral composition, bioaccumulation, and antioxidant enzyme function.

Graphical Abstract

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聚苯乙烯微塑料对淡水鱼（Labeo rohita）影响的新见解：对组织病理学、矿物质成分、生物累积性和抗氧化活性的评估

由于微塑料（MPs）在环境中的普遍存在以及在鱼类器官中的生物蓄积性，它们已成为一个亟待解决的问题，并可能威胁生态系统的健康。本研究通过探讨肠道组织病理学变化、肌肉中矿物质成分的改变、生物蓄积性和抗氧化酶活性的调节，研究了聚苯乙烯（PS）微塑料对鲮鱼（7.15 ± 0.02 克/尾）的影响。这项研究使用了六种试验日粮，包括不同聚苯乙烯微塑料（PS-MP）含量的葵花籽粉。在为期 90 天的喂养试验中，对照日粮（I）的聚苯乙烯微塑料含量为 0%，而 II-VI 日粮的聚苯乙烯微塑料含量分别为 0.5%、1%、1.5%、2% 和 2.5%。鱼类每天两次喂食各自的试验日粮，喂食量为每天湿体重的 5%。研究结果表明，摄入更多 PS-MPs 会大大改变抗氧化酶的活性和生物蓄积性。此外，组织病理学检查还发现肠道结构异常，并随着 PS-MPs 浓度的增加而恶化。值得注意的是，与对照组相比，试验日粮 VI（2.5% PS-MPs）显示雏鱼肌肉中的矿物质含量较低（P < 0.05）。总之，研究结果表明，PS-MPs 可能会对罗非鱼幼鱼产生负面影响，影响肠道组织病理学、矿物质成分、生物蓄积性和抗氧化酶的功能。

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来源期刊

Water, Air, & Soil Pollution 环境科学-环境科学

CiteScore

4.50

自引率

6.90%

发文量

448

审稿时长

2.6 months

期刊介绍： Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments. Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation. Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.