Mapping aged stem cell states associated with decline in skeletal muscle regeneration

Abstract

We used single-cell and high-resolution spatial transcriptomics to investigate age-related decline in mouse skeletal muscle regeneration. Using a transfer learning-based scoring approach, we identified a population of senescent-like muscle stem cells that increases with age and is induced by muscle injury. This highlights that halted stem cell self-renewal underlies the muscle regenerative dysfunction in mouse aging.