Association of RS1126478 (LTF) with molar-incisor and generalized periodontitis.

IF 3.1 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical Oral Investigations Pub Date : 2024-11-23 DOI:10.1007/s00784-024-06056-9

Camila S Stolf, Arthur G S Pereira, Bruna C Almeida, Hélvis E S Paz, Letícia S Arroteia, Hong Huang, Ikramuddin Aukhil, Peter Harrison, Manuela M V Miguel, Laís F F Ferraz, Mauro P Santamaria, Luciana M Shaddox, Renato C V Casarin

{"title":"Association of RS1126478 (LTF) with molar-incisor and generalized periodontitis.","authors":"Camila S Stolf, Arthur G S Pereira, Bruna C Almeida, Hélvis E S Paz, Letícia S Arroteia, Hong Huang, Ikramuddin Aukhil, Peter Harrison, Manuela M V Miguel, Laís F F Ferraz, Mauro P Santamaria, Luciana M Shaddox, Renato C V Casarin","doi":"10.1007/s00784-024-06056-9","DOIUrl":null,"url":null,"abstract":"Objectives: LTF SNP rs1126478 (T>C) could modulate Lactoferrin function and release and has been associated with periodontal disease in different locations before, but not in America. Thus, this study aimed to assess the association between this SNP and Grade C Periodontitis (Generalized (PerioC-G) and Molar Incisor Pattern (PerioC-MIP)) and seek a relationship between its presence and LTF gingival crevicular fluid (GCF) production.Material and methods: Oral cells from 361 Brazilians and 375 North Americans patients (Diseased and Health Controls (PH) from both locations) were collected. DNA was extracted, and a prefabricated probe system determined rs116478 genotyping. Immunoenzymatic analysis detected LTF levels in GCF.Results: Higher allelic altered-C frequency was associated with PerioC. For PerioC-G, CT and CC genotypes presented a frequency of 44.7% and 34.1%, respectively, while 37.3% and 26.4% were found to PH group (OD=2.1 (p=0.004) for CT and OD=2.3 (p=0.003) for CC). PerioC-G was associated with a lower concentration (742.2±717.9 pg/ml) of LTF than PerioC-MIP (5851±5859.2 pg/ml), p<0.0001. Multiple logistic regression showed that CC genotype impacted LTF levels by acting as a predictor (β =2.457, p=0.02, OD=1.17) despite the site or diagnosis.Conclusion: There is an association of rs1126478 with Grade C periodontitis, mainly the PerioC-G. Lower LTF levels in GCF might be associated with the generalized disease pattern.Clinical relevance: This study assessed and compared the genetic background in two distinct locations, paving the way for new studies regarding personalized risk and treatment for different ethnicities. As this SNP is in a region related to the antimicrobial function of LTF, it could impact PerioC-MIP and PerioC-G response against periodontopathogens. This discovery opens the way for in vitro studies analyzing the functionality of this SNP, and, in the future, it can be used to treat PerioC, individualizing the phenotypes.","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"28 12","pages":"653"},"PeriodicalIF":3.1000,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical Oral Investigations","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00784-024-06056-9","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives: LTF SNP rs1126478 (T>C) could modulate Lactoferrin function and release and has been associated with periodontal disease in different locations before, but not in America. Thus, this study aimed to assess the association between this SNP and Grade C Periodontitis (Generalized (PerioC-G) and Molar Incisor Pattern (PerioC-MIP)) and seek a relationship between its presence and LTF gingival crevicular fluid (GCF) production.

Material and methods: Oral cells from 361 Brazilians and 375 North Americans patients (Diseased and Health Controls (PH) from both locations) were collected. DNA was extracted, and a prefabricated probe system determined rs116478 genotyping. Immunoenzymatic analysis detected LTF levels in GCF.

Results: Higher allelic altered-C frequency was associated with PerioC. For PerioC-G, CT and CC genotypes presented a frequency of 44.7% and 34.1%, respectively, while 37.3% and 26.4% were found to PH group (OD=2.1 (p=0.004) for CT and OD=2.3 (p=0.003) for CC). PerioC-G was associated with a lower concentration (742.2±717.9 pg/ml) of LTF than PerioC-MIP (5851±5859.2 pg/ml), p<0.0001. Multiple logistic regression showed that CC genotype impacted LTF levels by acting as a predictor (β =2.457, p=0.02, OD=1.17) despite the site or diagnosis.

Conclusion: There is an association of rs1126478 with Grade C periodontitis, mainly the PerioC-G. Lower LTF levels in GCF might be associated with the generalized disease pattern.

Clinical relevance: This study assessed and compared the genetic background in two distinct locations, paving the way for new studies regarding personalized risk and treatment for different ethnicities. As this SNP is in a region related to the antimicrobial function of LTF, it could impact PerioC-MIP and PerioC-G response against periodontopathogens. This discovery opens the way for in vitro studies analyzing the functionality of this SNP, and, in the future, it can be used to treat PerioC, individualizing the phenotypes.

查看原文本刊更多论文

RS1126478 (LTF)与磨牙嵌合体和全身牙周炎的关系。

研究目的LTF SNP rs1126478 (T>C)可调节乳铁蛋白的功能和释放，以前曾在不同地区发现它与牙周病有关，但在美国没有发现。因此，本研究旨在评估该 SNP 与 C 级牙周炎（全身型（PerioC-G）和磨牙切牙型（PerioC-MIP））之间的关联，并寻求其存在与 LTF 龈沟液（GCF）分泌之间的关系：材料：收集了 361 名巴西患者和 375 名北美患者（两地的疾病对照和健康对照 (PH)）的口腔细胞。提取 DNA，用预制探针系统确定 rs116478 基因分型。免疫酶分析检测了 GCF 中的 LTF 水平：结果：较高的等位基因altered-C频率与PerioC有关。对于 PerioC-G，CT 和 CC 基因型的频率分别为 44.7% 和 34.1%，而 PH 组分别为 37.3% 和 26.4%（CT 的 OD=2.1 (p=0.004)，CC 的 OD=2.3 (p=0.003)）。与PerioC-MIP（5851±5859.2 pg/ml）相比，PerioC-G与较低的LTF浓度（742.2±717.9 pg/ml）相关：rs1126478与C级牙周炎（主要是PerioC-G）有关。GCF中较低的LTF水平可能与全身性疾病模式有关：这项研究对两个不同地区的遗传背景进行了评估和比较，为针对不同种族进行个性化风险和治疗的新研究铺平了道路。由于该 SNP 位于与 LTF 抗菌功能相关的区域，它可能会影响 PerioC-MIP 和 PerioC-G 对牙周病原体的反应。这一发现为分析该 SNP 功能的体外研究开辟了道路，将来它可用于治疗牙周病，使表型个体化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Clinical Oral Investigations 医学-牙科与口腔外科

CiteScore

6.30

自引率

5.90%

发文量

484

审稿时长

3 months

期刊介绍： The journal Clinical Oral Investigations is a multidisciplinary, international forum for publication of research from all fields of oral medicine. The journal publishes original scientific articles and invited reviews which provide up-to-date results of basic and clinical studies in oral and maxillofacial science and medicine. The aim is to clarify the relevance of new results to modern practice, for an international readership. Coverage includes maxillofacial and oral surgery, prosthetics and restorative dentistry, operative dentistry, endodontics, periodontology, orthodontics, dental materials science, clinical trials, epidemiology, pedodontics, oral implant, preventive dentistiry, oral pathology, oral basic sciences and more.