Nanodosimetric study of the γ-ray damage repair model based on the germ cell of Caenorhabditis elegans

IF 2.8 3区物理与天体物理 Q3 CHEMISTRY, PHYSICAL

Radiation Physics and Chemistry Pub Date : 2024-11-20 DOI:10.1016/j.radphyschem.2024.112415

Taotao Ji, Zhao Xu, Yuanyuan Zhou, Weiyue Yu, Taosheng Li, Yanyan Yang, Bing Hong

{"title":"Nanodosimetric study of the γ-ray damage repair model based on the germ cell of Caenorhabditis elegans","authors":"Taotao Ji, Zhao Xu, Yuanyuan Zhou, Weiyue Yu, Taosheng Li, Yanyan Yang, Bing Hong","doi":"10.1016/j.radphyschem.2024.112415","DOIUrl":null,"url":null,"abstract":"Monte Carlo calculations are extensively used in the fields of nanodosimetry and DNA damage research. Presently, the focus of DNA repair simulation studies is predominantly on the temporal aspects of the repair process, the impact of radiation dose on DNA repair requires further investigation. <ce:italic>Caenorhabditis elegans</ce:italic> (<ce:italic>C.elegans</ce:italic>) is one of the important model organisms in radiation biology research, and its germ cells are closely related to the DNA damage. Using the Hilbert curve, the DNA germ cell of <ce:italic>C. elegans</ce:italic> was constructed, and the double-strand breaks (DSB) yields quantified by Geant4-DNA. After comparing the simulation results of total DNA damage and cluster damage with biological data, it was found that the simulation results of cluster damage are close to the biological data. The number of DSBs induced by <ce:sup loc=\"post\">137</ce:sup>Cs radiation was further compared with experimental data at different doses and proposed a damage repair model for the number of DSBs in the germ cell of <ce:italic>C.elegans</ce:italic> that is related to the dose.The repair fitting was conducted for both total DSB damages and DSB cluster damages. After the application of the repair model fitting for the two types of DNA damage mentioned above, the results matched well with the corresponding biological experimental data (R<ce:sup loc=\"post\">2</ce:sup> > 0.950).","PeriodicalId":20861,"journal":{"name":"Radiation Physics and Chemistry","volume":"59 1","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Radiation Physics and Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.radphyschem.2024.112415","RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Monte Carlo calculations are extensively used in the fields of nanodosimetry and DNA damage research. Presently, the focus of DNA repair simulation studies is predominantly on the temporal aspects of the repair process, the impact of radiation dose on DNA repair requires further investigation. Caenorhabditis elegans (C.elegans) is one of the important model organisms in radiation biology research, and its germ cells are closely related to the DNA damage. Using the Hilbert curve, the DNA germ cell of C. elegans was constructed, and the double-strand breaks (DSB) yields quantified by Geant4-DNA. After comparing the simulation results of total DNA damage and cluster damage with biological data, it was found that the simulation results of cluster damage are close to the biological data. The number of DSBs induced by 137Cs radiation was further compared with experimental data at different doses and proposed a damage repair model for the number of DSBs in the germ cell of C.elegans that is related to the dose.The repair fitting was conducted for both total DSB damages and DSB cluster damages. After the application of the repair model fitting for the two types of DNA damage mentioned above, the results matched well with the corresponding biological experimental data (R2 > 0.950).

查看原文本刊更多论文

基于秀丽隐杆线虫生殖细胞的γ射线损伤修复模型的纳米模拟研究

蒙特卡洛计算被广泛应用于纳米模拟和 DNA 损伤研究领域。目前，DNA 修复模拟研究的重点主要集中在修复过程的时间方面，辐射剂量对 DNA 修复的影响还需要进一步研究。秀丽隐杆线虫（C.elegans）是辐射生物学研究的重要模式生物之一，其生殖细胞与DNA损伤密切相关。利用希尔伯特曲线构建了秀丽隐杆线虫的 DNA 生殖细胞，并通过 Geant4-DNA 对双链断裂（DSB）的产量进行了量化。将 DNA 总损伤和集束损伤的模拟结果与生物数据进行比较后发现，集束损伤的模拟结果与生物数据接近。进一步将 137Cs 辐射诱导的 DSB 数量与不同剂量下的实验数据进行了比较，并提出了秀丽隐杆线虫生殖细胞中 DSB 数量与剂量相关的损伤修复模型。对上述两种类型的DNA损伤进行修复模型拟合后，结果与相应的生物实验数据十分吻合（R2 > 0.950）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Radiation Physics and Chemistry 化学-核科学技术

CiteScore

5.60

自引率

17.20%

发文量

574

审稿时长

12 weeks

期刊介绍： Radiation Physics and Chemistry is a multidisciplinary journal that provides a medium for publication of substantial and original papers, reviews, and short communications which focus on research and developments involving ionizing radiation in radiation physics, radiation chemistry and radiation processing. The journal aims to publish papers with significance to an international audience, containing substantial novelty and scientific impact. The Editors reserve the rights to reject, with or without external review, papers that do not meet these criteria. This could include papers that are very similar to previous publications, only with changed target substrates, employed materials, analyzed sites and experimental methods, report results without presenting new insights and/or hypothesis testing, or do not focus on the radiation effects.