I V Podoprigora, M S Das, N B Zakharzhevskaya, L I Kafarskaya, B A Efimov
{"title":"Evaluation of the Biological Properties of the Bacteriodaceae Family Using the Caco-2 Cell Line.","authors":"I V Podoprigora, M S Das, N B Zakharzhevskaya, L I Kafarskaya, B A Efimov","doi":"10.1007/s10517-024-06281-y","DOIUrl":null,"url":null,"abstract":"<p><p>Disorders in the composition of the microbiota are often associated with pathological processes in the body. The use of new biotherapeutic drugs containing bacteria from normal microflora to correct these disorders may contribute to more effective treatment. The Bacteroidaceae family dominating in microflora is a candidate for inclusion in bacterial preparations. The adhesion properties of five bacterial strains from the Bacteroidaceae family were analyzed using the Caco-2 cell line and their effect on the transepithelial electrical resistance parameters (TEER) was assessed to determine the extent of their impact on the cell monolayer integrity. This approach allowed the selection of strain B. intestinalis 181, which was characterized by a moderate adhesion activity combined with a relatively low effect on the TEER parameter.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":""},"PeriodicalIF":0.9000,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Experimental Biology and Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10517-024-06281-y","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
引用次数: 0
Abstract
Disorders in the composition of the microbiota are often associated with pathological processes in the body. The use of new biotherapeutic drugs containing bacteria from normal microflora to correct these disorders may contribute to more effective treatment. The Bacteroidaceae family dominating in microflora is a candidate for inclusion in bacterial preparations. The adhesion properties of five bacterial strains from the Bacteroidaceae family were analyzed using the Caco-2 cell line and their effect on the transepithelial electrical resistance parameters (TEER) was assessed to determine the extent of their impact on the cell monolayer integrity. This approach allowed the selection of strain B. intestinalis 181, which was characterized by a moderate adhesion activity combined with a relatively low effect on the TEER parameter.
期刊介绍：
Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005.
World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells.
The Russian Volume Year is published in English from April.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
