Sex differences in the role of AKAP12 in behavioral function of middle-aged mice.

IF 4.9 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM
Hidehiro Ishikawa, Shintaro Kimura, Hajime Takase, Maximillian Borlongan, Norito Fukuda, Tomonori Hoshino, Gen Hamanaka, Ji Hyun Park, Akihiro Shindo, Kyu-Won Kim, Irwin H Gelman, Josephine Lok, Eng H Lo, Ken Arai
{"title":"Sex differences in the role of AKAP12 in behavioral function of middle-aged mice.","authors":"Hidehiro Ishikawa, Shintaro Kimura, Hajime Takase, Maximillian Borlongan, Norito Fukuda, Tomonori Hoshino, Gen Hamanaka, Ji Hyun Park, Akihiro Shindo, Kyu-Won Kim, Irwin H Gelman, Josephine Lok, Eng H Lo, Ken Arai","doi":"10.1186/s13293-024-00670-8","DOIUrl":null,"url":null,"abstract":"<p><p>A-kinase anchoring protein 12 (AKAP12) is a key scaffolding protein that regulates cellular signaling by anchoring protein kinase A (PKA) and other signaling molecules. While recent studies suggest an important role for AKAP12 in the brain, including cognitive functions, its role in middle-aged mice and potential sex differences are not fully understood. Therefore, this study investigated the effects of AKAP12 on cognitive and exploratory behavior in middle-aged mice, focusing on sex differences. Cognitive function was assessed using the spontaneous Y-maze test and the novel object recognition test (NORT). No significant sex differences in cognitive function were found in middle-aged C57BL/6J mice; however, female mice showed greater exploratory behavior during the NORT. In addition, both middle-aged male and female Akap12 knockout (KO) mice performed similarly to wild-type (WT) mice in the Y-maze test, but had lower discrimination indices in the NORT, suggesting a potential role for AKAP12 in short-term memory. Notably, exploratory behavior was suppressed in female Akap12 KO mice compared to WT mice, whereas male Akap12 KO mice did not show this effect. There were no significant differences in movement distance and velocity during the Y-maze test and NORT between WT and KO mice of either sex. These results indicate that AKAP12 affects cognitive function and exploratory behavior in middle-aged mice and that these effects differ between sexes.</p>","PeriodicalId":8890,"journal":{"name":"Biology of Sex Differences","volume":"15 1","pages":"93"},"PeriodicalIF":4.9000,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11580627/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biology of Sex Differences","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s13293-024-00670-8","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
引用次数: 0

Abstract

A-kinase anchoring protein 12 (AKAP12) is a key scaffolding protein that regulates cellular signaling by anchoring protein kinase A (PKA) and other signaling molecules. While recent studies suggest an important role for AKAP12 in the brain, including cognitive functions, its role in middle-aged mice and potential sex differences are not fully understood. Therefore, this study investigated the effects of AKAP12 on cognitive and exploratory behavior in middle-aged mice, focusing on sex differences. Cognitive function was assessed using the spontaneous Y-maze test and the novel object recognition test (NORT). No significant sex differences in cognitive function were found in middle-aged C57BL/6J mice; however, female mice showed greater exploratory behavior during the NORT. In addition, both middle-aged male and female Akap12 knockout (KO) mice performed similarly to wild-type (WT) mice in the Y-maze test, but had lower discrimination indices in the NORT, suggesting a potential role for AKAP12 in short-term memory. Notably, exploratory behavior was suppressed in female Akap12 KO mice compared to WT mice, whereas male Akap12 KO mice did not show this effect. There were no significant differences in movement distance and velocity during the Y-maze test and NORT between WT and KO mice of either sex. These results indicate that AKAP12 affects cognitive function and exploratory behavior in middle-aged mice and that these effects differ between sexes.

AKAP12 在中年小鼠行为功能中作用的性别差异。
A激酶锚定蛋白12(AKAP12)是一种关键的支架蛋白,它通过锚定蛋白激酶A(PKA)和其他信号分子来调节细胞信号传导。虽然最近的研究表明 AKAP12 在大脑(包括认知功能)中发挥着重要作用,但其在中年小鼠中的作用和潜在的性别差异尚未完全明了。因此,本研究调查了 AKAP12 对中年小鼠认知和探索行为的影响,重点关注性别差异。认知功能通过自发Y迷宫测试和新物体识别测试(NORT)进行评估。结果发现,C57BL/6J中年小鼠的认知功能没有明显的性别差异;但是,雌性小鼠在NORT中表现出更大的探索行为。此外,中年雄性和雌性Akap12基因敲除(KO)小鼠在Y迷宫测试中的表现与野生型(WT)小鼠相似,但在NORT中的辨别指数较低,这表明AKAP12在短时记忆中的潜在作用。值得注意的是,与WT小鼠相比,雌性Akap12 KO小鼠的探索行为受到抑制,而雄性Akap12 KO小鼠则没有这种影响。WT和KO小鼠在Y迷宫试验和NORT中的运动距离和速度没有明显差异。这些结果表明,AKAP12会影响中年小鼠的认知功能和探索行为,而且这些影响在性别之间存在差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Biology of Sex Differences
Biology of Sex Differences ENDOCRINOLOGY & METABOLISM-GENETICS & HEREDITY
CiteScore
12.10
自引率
1.30%
发文量
69
审稿时长
14 weeks
期刊介绍: Biology of Sex Differences is a unique scientific journal focusing on sex differences in physiology, behavior, and disease from molecular to phenotypic levels, incorporating both basic and clinical research. The journal aims to enhance understanding of basic principles and facilitate the development of therapeutic and diagnostic tools specific to sex differences. As an open-access journal, it is the official publication of the Organization for the Study of Sex Differences and co-published by the Society for Women's Health Research. Topical areas include, but are not limited to sex differences in: genomics; the microbiome; epigenetics; molecular and cell biology; tissue biology; physiology; interaction of tissue systems, in any system including adipose, behavioral, cardiovascular, immune, muscular, neural, renal, and skeletal; clinical studies bearing on sex differences in disease or response to therapy.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信