Transcriptome Analysis of HNSCC by Aggregatibacter actinomycetemcomitans Extracellular Vesicles.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2024-11-20 DOI:10.1111/odi.15204

Jae Yeong Ha, Song-Yi Choi, Suk-Jeong Kim, Kristen Jin Seog, Su-Hyung Hong, Heon-Jin Lee

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引用次数: 0

Abstract

Objectives: The role of extracellular vesicles (EVs), also known as outer membrane vesicles (OMVs), secreted by oral bacteria in the progression of head and neck squamous cell carcinomas (HNSCCs), is largely unexplored. This study aimed to investigate the influence of bacterial EVs, specifically those derived from Aggregatibacter actinomycetemcomitans (Aa), on the progression of HNSCC.

Materials and methods: FaDu and UMSCC1 cell lines were treated with Aa-derived EVs, and oncogenic activities were assessed. Comprehensive cellular and RNA-sequencing transcriptome analyses were conducted to assess the impact of these EVs on cell cycle progression and gene expression.

Results: Our findings reveal that Aa-derived EVs accelerate cell cycle progression through the S and G2 phases and enhance invasion in HNSCC cell lines. RNA-sequencing analysis showed that Aa-derived EVs exert a more significant effect on general transcript expression than on microRNA profiles, except for miR-146a, which is recognized as a key factor in both carcinogenesis and immune modulation.

Conclusions: Bacterial EVs, particularly from periodontal pathogens like Aa, are significant modulators within the oral cancer environment, potentially affecting cellular behavior and gene expression profiles. This study highlights the complex relationship between periodontal health and oral carcinogenesis, emphasizing the significant role of bacterial EVs in HNSCC progression.

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放线菌胞外小泡对 HNSCC 的转录组分析

研究目的口腔细菌分泌的细胞外囊泡 (EVs)，又称外膜囊泡 (OMVs)，在头颈部鳞状细胞癌 (HNSCC) 的进展过程中所起的作用在很大程度上尚未得到研究。本研究旨在探讨细菌EVs，特别是源自放线菌（Aa）的EVs对HNSCC进展的影响：用Aa衍生的EVs处理FaDu和UMSCC1细胞系，并评估其致癌活性。我们进行了全面的细胞和 RNA 序列转录组分析，以评估这些 EVs 对细胞周期进展和基因表达的影响：结果：我们的研究结果表明，Aa衍生的EV可加速细胞周期的进展，使其顺利通过S期和G2期，并增强HNSCC细胞系的侵袭能力。RNA序列分析表明，Aa衍生EVs对一般转录本表达的影响比对microRNA图谱的影响更显著，但miR-146a除外，后者被认为是致癌和免疫调节的关键因素：细菌EVs，尤其是来自牙周病原体（如Aa）的细菌EVs，是口腔癌环境中的重要调节剂，有可能影响细胞行为和基因表达谱。这项研究突出了牙周健康与口腔癌发生之间的复杂关系，强调了细菌 EV 在 HNSCC 进展中的重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.