Localization and expression of C1QTNF6 in chicken follicles and its regulatory effect on follicular granulosa cells

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2024-11-08 DOI:10.1016/j.psj.2024.104538

Chong Li , Qi Li , Xing Wu , Zihao Zhang , Jing Li , Ruirui Jiang , Guoxi Li , Xiaojun Liu , Xiangtao Kang , Zhuanjian Li , Donghua Li , Yadong Tian

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引用次数: 0

Abstract

C1q/tumor necrosis factor-related protein 6 (C1QTNF6) is a newly discovered adiponectin analog that plays a significant role in female reproduction. However, its expression and function in chickens remain unclear. In this study, the full-length coding sequence (CDS) of chicken C1QTNF6 was cloned from adult chicken ovary. We demonstrated that the putative C1QTNF6 protein shares a highly conserved amino acid sequence with known bird homologs. Using quantitative real-time PCR (qRT-PCR) and fluorescence in situ hybridization (FISH), we observed that C1QTNF6 is widely expressed in adult chicken follicle tissues, predominantly in granulosa cells (GCs). In vitro, stimulation with follicle-stimulating hormone (FSH) and luteinizing hormone (LH) significantly increased the expression of C1QTNF6 in chicken preovulatory granulosa cells (PoGCs) and prehierarchical granulosa cells (PhGCs), respectively. Overexpression and knockdown experiments in granulosa cells revealed that C1QTNF6 affects the proliferation and apoptosis of granulosa cells, reducing the expression of steroid hormone synthesis-related genes (STAR, Cyp11a1, Cyp19a1, and 3B-HSD) and hormones (progesterone and estrogen). In granulosa cells (GCs), co-treatment with C1QTNF6 and AdipoRon (an activator of adiponectin receptors) showed that AdipoRon promotes progesterone secretion, while C1QTNF6 inhibits AdipoR1 (adiponectin receptor 1) expression. There was no synergistic effect between C1QTNF6 and AdipoRon in steroid hormone production. Collectively, these findings suggest that C1QTNF6 may be a candidate gene for regulating follicular development and reproductive performance in chickens.

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C1QTNF6 在鸡卵泡中的定位和表达及其对卵泡颗粒细胞的调节作用。

C1q/肿瘤坏死因子相关蛋白6（C1QTNF6）是一种新发现的脂肪蛋白类似物，在雌性繁殖中发挥着重要作用。然而，它在鸡体内的表达和功能仍不清楚。本研究从成年鸡卵巢中克隆了鸡 C1QTNF6 的全长编码序列（CDS）。我们证明了推测的 C1QTNF6 蛋白与已知的鸟类同源物具有高度保守的氨基酸序列。利用实时定量 PCR（qRT-PCR）和荧光原位杂交（FISH），我们观察到 C1QTNF6 在成年鸡卵泡组织中广泛表达，主要是在颗粒细胞（GCs）中。在体外，卵泡刺激素（FSH）和黄体生成素（LH）分别显著增加了C1QTNF6在鸡排卵前颗粒细胞（PoGCs）和层前颗粒细胞（PhGCs）中的表达。在颗粒细胞中的过表达和敲除实验表明，C1QTNF6会影响颗粒细胞的增殖和凋亡，降低类固醇激素合成相关基因（STAR、Cyp11a1、Cyp19a1和3B-HSD）和激素（孕酮和雌激素）的表达。在颗粒细胞（GCs）中，用 C1QTNF6 和 AdipoRon（一种脂肪连接素受体激活剂）共同处理显示，AdipoRon 促进孕酮分泌，而 C1QTNF6 则抑制 AdipoR1（脂肪连接素受体 1）的表达。C1QTNF6 和 AdipoRon 在类固醇激素分泌方面没有协同作用。这些研究结果表明，C1QTNF6 可能是调节鸡卵泡发育和繁殖性能的候选基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.