Static Magnetic Stimulation and Magnetic Microwires Synergistically Enhance and Guide Neurite Outgrowth.

IF 10 2区 医学 Q1 ENGINEERING, BIOMEDICAL
Katelyn Neuman, Xiaoyu Zhang, Brian T Lejeune, Dominic Pizzarella, Manuel Vázquez, Laura H Lewis, Abigail N Koppes, Ryan A Koppes
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Abstract

Axonal growth is heavily influenced by topography and biophysical stimuli including magnetic and electrical fields. Despite extensive investigation, the degree of influence and the underlying genetic mechanisms remain poorly understood. Here, a novel approach to guide neurite growth is undertaken using an innovative ferromagnetic composite material - glass-coated magnetic microwire - to furnish a synergistic combination of magnetic and topographical cues. Whole rat dorsal root ganglia (DRG) are cultured under five different conditions: control, static magnetic field, magnetic microwire, static magnetic field + glass fiber, and static magnetic field + magnetic microwire. DRG outgrowth responses under each condition, including total neurite outgrowth and directionality, are compared. The combination of both magnetic stimulation and topography significantly increases total neurite outgrowth compared to the controls. The combination of magnetic stimulation and magnetic microwire lead to a strong directional bias of growth along the microwire, double what is observed with the glass fiber. Next generation RNA sequencing of DRG exposed to static magnetic field + magnetic microwire reveals the downregulation of genes relating to the immune response, interleukin signaling, and signal transduction. These results set the stage for contemplating future biophysical stimulation for axonal guidance and improved understanding of material-tissue interactions.

静态磁刺激与磁微线协同增强并引导神经元生长
轴突生长在很大程度上受地形和生物物理刺激(包括磁场和电场)的影响。尽管进行了广泛的研究,但对其影响程度和潜在的遗传机制仍然知之甚少。在这里,我们采用了一种创新的铁磁复合材料--玻璃涂层磁性微线--来提供磁性和地形线索的协同组合,从而引导神经元生长。整个大鼠背根神经节(DRG)在五种不同条件下进行培养:对照组、静态磁场、磁性微线、静态磁场 + 玻璃纤维和静态磁场 + 磁性微线。比较了每种条件下 DRG 的生长反应,包括神经元的总生长量和方向性。与对照组相比,磁刺激和地形图的组合能显著增加神经元的总生长量。磁刺激和磁性微线的结合会导致神经元沿微线生长的强烈方向性偏差,是玻璃纤维所观察到的偏差的两倍。对暴露于静态磁场和磁性微线的 DRG 进行的下一代 RNA 测序显示,与免疫反应、白细胞介素信号转导和信号转导有关的基因出现下调。这些结果为未来考虑轴突导向的生物物理刺激和更好地理解材料与组织之间的相互作用奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Advanced Healthcare Materials
Advanced Healthcare Materials 工程技术-生物材料
CiteScore
14.40
自引率
3.00%
发文量
600
审稿时长
1.8 months
期刊介绍: Advanced Healthcare Materials, a distinguished member of the esteemed Advanced portfolio, has been dedicated to disseminating cutting-edge research on materials, devices, and technologies for enhancing human well-being for over ten years. As a comprehensive journal, it encompasses a wide range of disciplines such as biomaterials, biointerfaces, nanomedicine and nanotechnology, tissue engineering, and regenerative medicine.
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