Plant PI4P is required for bacteria to translocate type-3 effectors

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2024-11-20 DOI:10.1111/nph.20248

Jinfeng Peng, Liyuan Zhang, Kai Lu, Xiaochen Chen, Hao Pang, Xiaohui Yao, Ping Li, Peng Cao, Xiaoxu Li, Zuodong Wang, Lina Qin, Miao Zhou, Maoling Wang, Qizhen Li, Chunyu Qiu, Mingxin Sun, Yufen Li, Liping Gong, Xinlin Wei, Siyi Wang, Jiajia Chen, Chongchong Lu, Shenshen Zou, Xinhua Ding, Lei Chen, Meixiang Zhang, Hansong Dong

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引用次数: 0

Abstract

Type-3 effectors (T3E) of phytopathogenic Gram-negative bacteria fulfill a virulent role, causing disease, or an avirulent role, inducing immunity, following their translocation into plant cells. This study aimed to validate the hypothesis that bacterial T3E translocation requires lipidic compounds in plant cell membranes.
Based on genetic, molecular, and biochemical assays, we determined that phosphatidylinositol 4-phosphate (PI4P) associated with plant cell membranes is essential for the translocation of T3E by bacterial pathogens.
Replicate experimental data revealed that PI4P cooperates with the type-3 translocase HrpF to facilitate the translocation of effectors TAL and Xop from Xanthomonas oryzae and Hop from Pseudomonas syringae into the cells of Oryza sativa and Nicotiana benthamiana, respectively. Genetic and molecular analyses confirmed that, once translocated into plant cells, the distinct effectors induce disease or immunity. Combined genetic and pharmacological analyses revealed that when PI4P content is suppressed via genetic or pharmacological measures, the T3 effector translocation is considerably suppressed, resulting in serious inhibition of bacterial infection.
Overall, these findings demonstrate that cooperative functioning of HrpF–PI4P is conserved in bacterial effectors and plants.

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细菌转运 3 型效应物需要植物 PI4P

植物病原革兰氏阴性细菌的 3 型效应物（T3E）转入植物细胞后，可发挥致病或诱导免疫的作用。本研究旨在验证细菌 T3E 转位需要植物细胞膜中脂质化合物的假设。基于遗传、分子和生化实验，我们确定与植物细胞膜相关的磷脂酰肌醇 4-磷酸（PI4P）是细菌病原体转运 T3E 的必要条件。重复的实验数据显示，PI4P 与 3 型转运酶 HrpF 合作，分别促进黄单胞菌的 TAL 和 Xop 以及假单胞菌的 Hop 的效应物转运到水稻和烟草的细胞中。遗传和分子分析证实，这些不同的效应物一旦转入植物细胞，就会诱发疾病或免疫。遗传学和药理学的综合分析表明，当通过遗传学或药理学措施抑制 PI4P 含量时，T3 效应子的转运将受到极大抑制，从而严重抑制细菌感染。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.