ImmunoCellCycle-ID - a high-precision immunofluorescence-based method for cell cycle identification.

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Journal of cell science Pub Date : 2024-11-15 Epub Date: 2024-11-20 DOI:10.1242/jcs.263414
Yu-Lin Chen, Yu-Chia Chen, Aussie Suzuki
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引用次数: 0

Abstract

The cell cycle is a fundamental process essential for cell proliferation, differentiation and development. It consists of four major phases: G1, S, G2 and M. These phases collectively drive the reproductive cycle and are meticulously regulated by various proteins that play crucial roles in both the prevention and progression of cancer. Traditional methods for studying these functions, such as flow cytometry, require a substantial number of cells to ensure accuracy. In this study, we have developed a user-friendly immunofluorescence-based method for identifying cell cycle stages, providing single-cell resolution and precise identification of G1, early/mid S, late S, early/mid G2, late G2, and each sub-stage of the M phase using fluorescence microscopy called ImmunoCellCycle-ID. This method provides high-precision cell cycle identification and can serve as an alternative to, or in combination with, traditional flow cytometry to dissect detailed sub-stages of the cell cycle in a variety of cell lines.

ImmunoCellCycle-ID--一种基于免疫荧光的高精度细胞周期鉴定方法。
细胞周期是细胞增殖、分化和发育必不可少的基本过程。它由四个主要阶段组成:G1、S、G2 和 M:G1、S、G2 和 M 四个阶段共同驱动着生殖周期,并受到各种蛋白质的严格调控,这些蛋白质在癌症的预防和发展过程中发挥着至关重要的作用。研究这些功能的传统方法(如流式细胞术)需要大量细胞才能确保准确性。在这项研究中,我们开发了一种用户友好型基于免疫荧光的细胞周期阶段鉴定方法,利用荧光显微镜提供单细胞分辨率并精确鉴定 G1、早/中 S 期、晚 S 期、早/中 G2 期、晚 G2 期和 M 期的每个亚阶段,称为 ImmunoCellCycle-ID。这种方法可提供高精度的细胞周期鉴定,可替代传统的流式细胞术或与之结合使用,在各种细胞系中剖析细胞周期的详细亚阶段。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of cell science
Journal of cell science 生物-细胞生物学
CiteScore
7.30
自引率
2.50%
发文量
393
审稿时长
1.4 months
期刊介绍: Journal of Cell Science publishes cutting-edge science, encompassing all aspects of cell biology.
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