Exploring differential miRNA expression profiles in muscular and visceral adipose tissue of patients with severe obesity.

IF 4.2 2区医学 Q1 ENDOCRINOLOGY & METABOLISM

International Journal of Obesity Pub Date : 2024-11-19 DOI:10.1038/s41366-024-01683-4

Carmen Lambert, Paula Morales-Sánchez, Ana Victoria García, Elsa Villa-Fernández, Jèssica Latorre, Miguel García-Villarino, Estrella Olga Turienzo Santos, Lorena Suárez-Gutierrez, Raquel Rodríguez Uría, Sandra Sanz Navarro, Jessica Ares-Blanco, Pedro Pujante, Lourdes María Sanz Álvarez, Edelmiro Menéndez-Torre, María Moreno Gijón, José Manuel Fernandez-Real, Elías Delgado

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引用次数: 0

Abstract

Background: This study aims to investigate the differential miRNA expression profile between the visceral white adipose tissue and the skeletal muscle of people with obesity undergoing bariatric surgery.

Methods: Skeletal muscle and visceral adipose tissue samples of 10 controls and 38 people with obesity (50% also with type 2 diabetes) undergoing bariatric surgery were collected. miRNA expression profiles were analyzed using Next-Generation Sequencing and subsequently validated using RT-PCR.

Results: Approximately 69% of miRNAs showed similar expression in both tissues, however, 55 miRNAs were preferentially expressed in visceral adipose tissue and 53 in skeletal muscle. miR-122b-5p was uniquely identified in skeletal muscle, while miR-1-3p and miR-206 were upregulated in skeletal muscle. Conversely, miR-224-5p and miR-335-3p exhibited upregulation in visceral adipose tissue. Notably, distinctions related to the presence of type 2 diabetes were observed solely in the expression of miR-1-3p and miR-206 in visceral adipose tissue.

Conclusions: This is the first study unveiling distinct miRNA expression profiles in paired samples of visceral adipose tissue and skeletal muscle in humans. The identification of obesity-specific miRNAs in these tissues opens up promising avenues for research into potential biomarkers for obesity diagnosis and treatment.

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探索重度肥胖症患者肌肉和内脏脂肪组织中不同的 miRNA 表达谱。

研究背景本研究旨在调查接受减肥手术的肥胖症患者的内脏白色脂肪组织和骨骼肌之间不同的 miRNA 表达谱：方法：收集 10 名对照组和 38 名接受减肥手术的肥胖症患者（50% 同时患有 2 型糖尿病）的骨骼肌和内脏脂肪组织样本：然而，55 个 miRNA 在内脏脂肪组织中优先表达，53 个在骨骼肌中优先表达。miR-122b-5p 在骨骼肌中唯一被识别，而 miR-1-3p 和 miR-206 在骨骼肌中上调。相反，miR-224-5p 和 miR-335-3p 在内脏脂肪组织中表现出上调。值得注意的是，仅在内脏脂肪组织中观察到 miR-1-3p 和 miR-206 的表达与是否患有 2 型糖尿病有关：这是首次揭示人类内脏脂肪组织和骨骼肌配对样本中不同 miRNA 表达谱的研究。这些组织中肥胖特异性 miRNA 的鉴定为研究肥胖诊断和治疗的潜在生物标记物开辟了一条前景广阔的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Obesity 医学-内分泌学与代谢

CiteScore

10.00

自引率

2.00%

发文量

221

审稿时长

3 months

期刊介绍： The International Journal of Obesity is a multi-disciplinary forum for research describing basic, clinical and applied studies in biochemistry, physiology, genetics and nutrition, molecular, metabolic, psychological and epidemiological aspects of obesity and related disorders. We publish a range of content types including original research articles, technical reports, reviews, correspondence and brief communications that elaborate on significant advances in the field and cover topical issues.