Exogenous silicon improved the cell wall stability by activating non-structural carbohydrates and structural carbohydrates metabolism in salt and drought stressed Glycyrrhiza uralensis stem.

Abstract

The plant cell wall is a crucial barrier against environmental stress, mainly composed of lignin and carbohydrates such as cellulose, hemicellulose, and pectin. This study explored the direct regulatory mechanism of silicon (Si) on cell wall components of Glycyrrhiza uralensis (G. uralensis) stems under salt and drought (S + D) stress and the indirect regulatory mechanism of non-structural carbohydrates on structural carbohydrates, mediated by uridine diphosphate glucose (UDPG), through joint physiological, biochemical, and transcriptomic analyses. Under S + D stress, Si increased the contents of cell wall components, altered the structure of cell wall, and directly promoted cell wall re-construction by regulating gene expression levels and enzyme activities related to cell wall biosynthesis. Meanwhile, Si facilitated the accumulation of carbohydrates by regulating enzyme activities and gene expression levels in the anabolic pathway of polysaccharides, thereby promoting UDPG conversion and indirectly providing substrates for cell wall synthesis. In conclusion, Si directly and indirectly facilitates the synthesis of cell wall components by regulating both cell wall metabolism and non-structural carbohydrates metabolism, thus reinforcing the cell wall, enhancing its stability, and improving the salt and drought tolerance of G. uralensis stems.