Antisense transcription is associated with expression of metal resistance determinants in Cupriavidus metallidurans CH34.

IF 2.9 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Metallomics Pub Date : 2024-12-02 DOI:10.1093/mtomcs/mfae057
Cornelia Große, Jan Grau, Martin Herzberg, Dietrich H Nies
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引用次数: 0

Abstract

Cupriavidus metallidurans is able to thrive in metal-rich environments but also survives metal starvation. Expression of metal resistance determinants in C. metallidurans was investigated on a global scale. Cupriavidus metallidurans was challenged with a MultiTox metal mix specifically designed for the wildtype strain CH34 and its plasmid-free derivative AE104, including treatment with ethylenediamintetraacetate (EDTA), or without challenge. The sense and antisense transcripts were analyzed in both strains and under all three conditions by RNASeq. A total of 10 757 antisense transcripts (ASTs) were assigned to sense signals from genes and untranslated regions, and 1 319 of these ASTs were expressed and were longer than 50 bases. Most of these (82%) were dual-use transcripts that contained antisense and sense regions, but ASTs (16%) were also observed that had no sense regions. Especially in metal-treated cells of strains CH34 and AE104, up- or down-regulated sense transcripts were accompanied by antisense transcription activities that were also regulated. The presence of selected asRNAs was verified by reverse transcription polymerase chain reaction (RT-PCR). Following metal stress, expression of genes encoding components of the respiratory chain, motility, transcription, translation, and protein export were down-regulated. This should also affect the integration of the metal efflux pumps into the membrane and the supply of the energy required to operate them. To solve this dilemma, transcripts for the metal efflux pumps may be stabilized by interactions with ASTs to allow their translation and import into the membrane. Alternatively, metal stress possibly causes recruitment of RNA polymerase from housekeeping genes for preferential expression of metal resistance determinants.

反义转录与Cupriavidus metallidurans CH34中抗金属决定因子的表达有关。
铜绿微囊藻(Cupriavidus metallidurans)能够在富含金属的环境中茁壮成长,也能在金属饥饿状态下存活。我们在全球范围内调查了金属铜绿微囊藻中金属抗性决定因子的表达情况。用专门为野生型菌株 CH34 及其无质粒衍生物 AE104 设计的 MultiTox 金属混合物(包括乙二胺四乙酸盐(EDTA)处理)对金属栗蝇进行挑战,或不进行挑战。通过 RNASeq 分析了这两种菌株在所有三种条件下的有义和反义转录本。共有 10 757 个反义转录本(AST)被分配到来自基因和非翻译区的有义信号,其中 1 319 个反义转录本表达且长度超过 50 个碱基。其中大部分(82%)是包含反义和有义区域的两用转录本,但也观察到没有有义区域的 ASTs(16%)。特别是在经金属处理的 CH34 和 AE104 菌株细胞中,上调或下调的有义转录本也伴随着反义转录活性的调节。通过 RT-PCR 验证了所选 asRNA 的存在。金属胁迫后,编码呼吸链、运动、转录、翻译和蛋白质输出成分的基因表达下调。这也会影响金属外排泵与膜的整合以及运行所需的能量供应。为解决这一难题,金属外排泵的转录本可能会通过与 ASTs 的相互作用而稳定下来,使其能够翻译并导入膜中。另外,金属应激也可能导致从看家基因中招募 RNA 聚合酶,以优先表达金属抗性决定因子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Metallomics
Metallomics 生物-生化与分子生物学
CiteScore
7.00
自引率
5.90%
发文量
87
审稿时长
1 months
期刊介绍: Global approaches to metals in the biosciences
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