Enhanced red emission of upconversion nanoparticles via Li+ and Tm3+ codoping and active core-shell construction for sensitive detection of miRNAs

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2024-11-17 DOI:10.1016/j.aca.2024.343429

Yingchao Li, Canzhao Tu, Qianshun Chen, Yingying Lin, Baoming Li, Haixia Lyu

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Abstract

The overexpression of microRNA-222 (miRNA-222) is closely related to many human diseases, so the development of biosensors to detect this biomarker will contribute to the diagnosis of related diseases. Here, a simple, sensitive and specific fluorescence assay for the detection of miRNA-222 was developed using red-emitting upconversion nanoparticle (UCNP) as the donor and a DNA hairpin with black hole quencher-2 (BHQ-2) as the acceptor. Li⁺ and Tm³⁺-doped UCNP with a strong emission peak at 654 nm was obtained by changing the doped ion ratio and constructing core-shell structures. Under optimal conditions, the linear range for detecting miRNA-222 is 0.5∼2.5 nM and the limit of detection is as low as 0.077 nM without any complicated amplification strategy. Finally, the proposed assay was applied for the detection of miRNA-222 in serum samples. The results obtained were similar to those of the standard method, and the spiked recoveries were in the range of 97.62%-102.14%, suggesting that the proposed method has practical value in a complex biological sample matrix.

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通过 Li+ 和 Tm3+ 共掺以及活性核壳结构增强上转换纳米粒子的红色发射，实现 miRNA 的灵敏检测

microRNA-222（miRNA-222）的过度表达与许多人类疾病密切相关，因此开发检测该生物标志物的生物传感器将有助于相关疾病的诊断。本文以红色发光的上转换纳米粒子（UCNP）为供体，以带有黑洞淬灭剂-2（BHQ-2）的DNA发夹为受体，开发了一种简单、灵敏和特异的荧光检测miRNA-222的方法。通过改变掺杂离子比例和构建核壳结构，获得了掺杂 Li+ 和 Tm3+ 的 UCNP，并在 654 纳米波长处获得了强发射峰。在最佳条件下，检测 miRNA-222 的线性范围为 0.5∼2.5 nM，检测限低至 0.077 nM，无需复杂的扩增策略。最后，该方法被用于检测血清样本中的 miRNA-222。结果与标准方法相似，加标回收率在97.62%-102.14%之间，表明该方法在复杂的生物样品基质中具有实用价值。

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.