Appropriate water and nitrogen supply regulates the dynamics of nitrogen translocation and thereby enhancing the accumulation of nitrogen in maize grains

Abstract

To improve nitrogen uptake and grain quality in maize, this study explores the dynamic processes of nitrogen accumulation, distribution, and translocation under varying water and nitrogen supplies, aiming to optimize water-nitrogen management practices. Field trials were conducted in Karamay, Xinjiang, in 2022 and 2023, with different irrigation levels (75 % ET_c, 100 % ET_c, 125 % ET_c) and nitrogen application rates (0, 93, 186, 279 kg Nhm⁻²). The effects of water and nitrogen supply on nitrogen accumulation and distribution in aboveground maize organs were analyzed, and the dynamic characteristics of maize nitrogen accumulation were examined using the characteristic parameters of the Richards nitrogen accumulation equation. The results showed that beyond the W2N2 treatment (irrigation at 100 % ET_c and nitrogen application of 186 kg N hm⁻²), increases in irrigation and nitrogen did not significantly enhance nitrogen accumulation per plant. Under W2N2, high levels of nitrogen were accumulated in maize leaf, stem, bract, cob, and grain. The nitrogen transfer among different organs and their contribution to grain nitrogen showed the following hierarchy: leaf > stem > cob > bract, with the contribution rates to grain nitrogen ranging from 26.16 % to 56.23 % over the two years. The Richards model accurately quantified the dynamic relationship between water-nitrogen supply and crop nitrogen accumulation, with the coefficient of determination (R²) ranging from 0.9864 to 0.9999 and the normalized root mean square error (NRMSE) from 0.70 % to 6.51 %. Optimal water-nitrogen supply significantly reduced the accumulated temperature required for maize to enter the rapid nitrogen accumulation phase and achieve maximum growth rates, while extending the duration of the rapid growth phase and increasing both the maximum growth rate and the average growth rate during this period. Grain nitrogen accumulation was positively correlated with nitrogen accumulation rates, as well as nitrogen accumulation and translocation in various organs. Under suitable irrigation and nitrogen application, the interactive effects of water and nitrogen (W × N) significantly increased both nitrogen accumulation and nitrogen accumulation rates, laying a foundation for nitrogen translocation to grains in the late growth stages and enhancing grain nitrogen accumulation. Thus, appropriate water and nitrogen supply can significantly influence nitrogen accumulation, distribution, and translocation processes in maize, regulating grain nitrogen accumulation. This study provides valuable information for nitrogen accumulation regulation and grain quality improvement in maize in Xinjiang and other regions with similar climatic conditions.