Species and genetic diversity of notorious dinoflagellates Pfiesteria piscicida, Luciella masanensis, and relatives in marine sediments of China

IF 5.5 1区 生物学 Q1 MARINE & FRESHWATER BIOLOGY
Zhe Tao , Xiaohan Liu , Xiaoying Song , Yunyan Deng , Lixia Shang , Zhaoyang Chai , Zhangxi Hu , Yuyang Liu , Ying Zhong Tang
{"title":"Species and genetic diversity of notorious dinoflagellates Pfiesteria piscicida, Luciella masanensis, and relatives in marine sediments of China","authors":"Zhe Tao ,&nbsp;Xiaohan Liu ,&nbsp;Xiaoying Song ,&nbsp;Yunyan Deng ,&nbsp;Lixia Shang ,&nbsp;Zhaoyang Chai ,&nbsp;Zhangxi Hu ,&nbsp;Yuyang Liu ,&nbsp;Ying Zhong Tang","doi":"10.1016/j.hal.2024.102746","DOIUrl":null,"url":null,"abstract":"<div><div>The dinoflagellate genus <em>Pfiesteria</em>, encompassing <em>Pfiesteria piscicida</em> and <em>P. shumwayae</em>, became a hot topic in HABs research in the early 2000s due to numerous but controversial reports regarding life cycle and toxicity. While <em>Pfiesteria</em> presence has been reported from all continents, surprisingly, there has been no documented presence in China to date. Here, we report our findings on the presence, species, and genetic diversity of <em>Pfiesteria</em> and its phylogenetic relatives (particularly <em>Luciella masanensis</em>) using a combined approach. First, we demonstrated the presence of <em>P. piscicida</em> and <em>L. masanensis</em> using metabarcoding analysis of &gt;320 sediment samples. <em>Pfiesteria piscicida</em> was identified in 32 sampling sites across all four seas of China, with rDNA sequences exhibiting considerable differences from the type strain (up to 3.83 %), while <em>Luciella masanensis</em> presented in 212 sites from all four seas and included only ribotypes 1 and 3 among the four known ribotypes. Second, based on the metabarcoding detections, our application of FISH with species-specific probes and subsequent single-cyst PCR sequencing to the “positive” sediments confirmed, both morphologically and molecularly, the existence of <em>P. piscicida</em> and <em>L. masanensis</em> cysts in the sediments. Finally, individual cysts were isolated using the sodium polytungstate protocol and sequenced targeting 28S rDNA D1–D6 domains. Phylogenetic analysis revealed that 35 resting cysts were relatives of <em>Pfiesteria</em>, likely belonging to either new species or novel genera and family because they formed at least two distinct clades in the phylogenetic tree. <em>Pfiesteria shumwayae</em> was not detected from any sample, suggesting its absence in Chinese waters. We believe this work provides important factual basis for the global biogeography of these species and future HABs monitoring of in China.</div></div>","PeriodicalId":12897,"journal":{"name":"Harmful Algae","volume":"140 ","pages":"Article 102746"},"PeriodicalIF":5.5000,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Harmful Algae","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1568988324001793","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

The dinoflagellate genus Pfiesteria, encompassing Pfiesteria piscicida and P. shumwayae, became a hot topic in HABs research in the early 2000s due to numerous but controversial reports regarding life cycle and toxicity. While Pfiesteria presence has been reported from all continents, surprisingly, there has been no documented presence in China to date. Here, we report our findings on the presence, species, and genetic diversity of Pfiesteria and its phylogenetic relatives (particularly Luciella masanensis) using a combined approach. First, we demonstrated the presence of P. piscicida and L. masanensis using metabarcoding analysis of >320 sediment samples. Pfiesteria piscicida was identified in 32 sampling sites across all four seas of China, with rDNA sequences exhibiting considerable differences from the type strain (up to 3.83 %), while Luciella masanensis presented in 212 sites from all four seas and included only ribotypes 1 and 3 among the four known ribotypes. Second, based on the metabarcoding detections, our application of FISH with species-specific probes and subsequent single-cyst PCR sequencing to the “positive” sediments confirmed, both morphologically and molecularly, the existence of P. piscicida and L. masanensis cysts in the sediments. Finally, individual cysts were isolated using the sodium polytungstate protocol and sequenced targeting 28S rDNA D1–D6 domains. Phylogenetic analysis revealed that 35 resting cysts were relatives of Pfiesteria, likely belonging to either new species or novel genera and family because they formed at least two distinct clades in the phylogenetic tree. Pfiesteria shumwayae was not detected from any sample, suggesting its absence in Chinese waters. We believe this work provides important factual basis for the global biogeography of these species and future HABs monitoring of in China.
中国海洋沉积物中臭名昭著的甲藻 Pfiesteria piscicida、Luciella masanensis 及其近缘种的种类和遗传多样性
本世纪初,由 Pfiesteria piscicida 和 P. shumwayae 组成的甲藻属 Pfiesteria 成为有害藻华研究的热门话题,因为有关其生命周期和毒性的报告很多,但却存在争议。虽然各大洲都有关于 Pfiesteria 存在的报道,但令人惊讶的是,迄今为止还没有关于中国存在 Pfiesteria 的记录。在此,我们采用一种综合方法,报告了我们对 Pfiesteria 及其系统发育近缘种(尤其是 Masanensis Luciella)的存在、种类和遗传多样性的研究结果。首先,我们通过对 320 份沉积物样本进行代谢编码分析,证明了 P. piscicida 和 L. masanensis 的存在。Pfiesteria piscicida在中国所有四个海域的32个采样点被鉴定,其rDNA序列与模式株有相当大的差异(高达3.83%);而Luciella masanensis在所有四个海域的212个采样点被鉴定,在已知的四个核型中仅包括核型1和核型3。其次,在元条码检测的基础上,我们对 "阳性 "沉积物使用物种特异性探针进行 FISH 检测,并随后进行单囊蚴 PCR 测序,从形态学和分子学上证实了 P. piscicida 和 L. masanensis 囊蚴在沉积物中的存在。最后,使用多钨酸钠方案分离了单个囊蚴,并对 28S rDNA D1-D6 域进行了测序。系统进化分析表明,35 个静止孢囊是 Pfiesteria 的近亲,很可能属于新种或新属和新科,因为它们在系统进化树中至少形成了两个不同的支系。在所有样本中都没有检测到 Pfiesteria shumwayae,这表明中国水域中没有这种细菌。我们相信,这项工作为这些物种的全球生物地理学和中国未来的有害藻华监测提供了重要的事实依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Harmful Algae
Harmful Algae 生物-海洋与淡水生物学
CiteScore
12.50
自引率
15.20%
发文量
122
审稿时长
7.5 months
期刊介绍: This journal provides a forum to promote knowledge of harmful microalgae and macroalgae, including cyanobacteria, as well as monitoring, management and control of these organisms.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信