The interaction effect of water deficit stress and nanosilicon on phytochemical and physiological characteristics of hemp (Cannabis sativa L.)

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-11-15 DOI:10.1016/j.plaphy.2024.109298

Ayyub Rezghiyan , Hassan Esmaeili , Mohsen Farzaneh , Hassan Rezadoost

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引用次数: 0

Abstract

Different practical approaches have been employed to attenuate the destructive impacts of water deficit stress on plants, such as utilization of humic acid, salicylic acid, algae extract, mulching, and microorganisms, as well as silicon application. Nanosilicon significantly moderates the ruinous effects of abiotic and biotic stress in plants through some physiological processes. In this study, the interaction effect of drought stress and nanosilicon on phytochemical and physiological characteristics of hemp (Cannabis sativa L.) was investigated, wherein the four-week-old seedlings were subjected to irrigation treatments at four levels, including 100% (control), 80% (mild stress), 60% (moderate stress), and 40% (severe stress) of field capacity and nanosilicon at three concentrations (0, 0.5, and 1.5 mM) was foliar applied every 10 days in a factorial completely randomized design experiment with three replications for 30 days. Phytochemical and physiological analyses such as photosynthetic pigments, total phenolic and flavonoid content, and antioxidant enzyme activities were conducted. The results indicated that the highest content of Cannabidiol and Tetrahydrocannabinol was achieved using 1.5 mM (1.89%) and 0.5 mM (0.63%) nanosilicon treatments, respectively, under moderate stress. The plants subjected to severe drought stress without nanosilicon application displayed the lowest values of chlorophyll a (0.50 mg/g FW) and b (0.20 mg/g FW). The use of nanosilicon excited the activation of antioxidant enzymes, wherein the plants treated with nanosilicon and drought stress exhibited significantly higher SOD, POD, and APX activities compared to the control. Under all drought stress levels, foliar application of nanosilicon at the highest concentration decreased proline content. The results proposed that the application of 1.5 mM nanosilicon, as a more efficient concentration, improved drought tolerance in hemp plants.

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缺水胁迫和纳米硅对大麻（Cannabis sativa L.）植物化学和生理特性的交互效应

人们采用了不同的实用方法来减轻缺水胁迫对植物的破坏性影响，如利用腐植酸、水杨酸、藻类提取物、覆盖物和微生物以及施硅。纳米硅可通过一些生理过程明显缓和非生物和生物胁迫对植物的破坏性影响。本研究探讨了干旱胁迫和纳米硅对大麻（Cannabis sativa L. ）植物化学和生理特性的交互作用。研究采用完全随机设计的因子实验，每隔 10 天叶面喷施一次三种浓度（0、0.5 和 1.5 mM）的纳米硅，每次喷施量为田间灌溉能力的 100%（对照）、80%（轻度胁迫）、60%（中度胁迫）和 40%（重度胁迫），连续喷施 30 天。进行了植物化学和生理分析，如光合色素、总酚和类黄酮含量以及抗氧化酶活性。结果表明，在中度胁迫下，使用 1.5 mM（1.89%）和 0.5 mM（0.63%）纳米硅处理的大麻二酚和四氢大麻酚含量最高。在未施用纳米硅的情况下，受到严重干旱胁迫的植物叶绿素 a（0.50 mg/g FW）和叶绿素 b（0.20 mg/g FW）值最低。纳米硅的使用促进了抗氧化酶的活化，与对照组相比，使用纳米硅和干旱胁迫处理的植物表现出更高的 SOD、POD 和 APX 活性。在所有干旱胁迫水平下，叶面喷施最高浓度的纳米硅可降低脯氨酸含量。结果表明，施用 1.5 mM 纳米硅作为一种更有效的浓度，可提高大麻植物的耐旱性。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.