Embarking on a journey through Micro-RNA and Circular-RNA detection methods

Abstract

Micro-RNAs (miRNAs) and circular-RNAs (circRNAs) are attracting attention as biomarkers for cancer diagnosis due to their distinct biological characteristics. MiRNAs, known for their critical role in gene regulation and tissue specificity, face challenges in detection due to their small size and low abundance in clinical samples. CircRNAs, on the other hand, offer advantages in stability based on their circular structure and are emerging as robust biomarkers. Traditional detection methods have limitations in sensitivity and specificity. Recent developments in isothermal amplification methods promise improved detection capabilities. This review focuses on the innovative primer/probe designs, which enhanced the efficiency of miRNA and circRNA detection in real samples.