Investigating the esterification-induced inhibition in enzymatic saccharification of corn stover pretreated with carboxylic-acid based deep eutectic solvent

IF 5.6 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products Pub Date : 2024-11-12 DOI:10.1016/j.indcrop.2024.120031

Liyi Zhang , Guangyong Zeng , Benkun Qi , Jianquan Luo , Yinhua Wan

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引用次数: 0

Abstract

Choline chloride-lactic acid, a common carboxylic acid-based deep eutectic solvent (DES), was used to pretreat corn stover (CS). Despite significant lignin and xylan removal, enzymatic cellulose conversion remained below 30 %, only slightly higher than untreated CS. Structural characterization confirmed esterification between the CS's hydroxyl groups and lactic acid during pretreatment. A brief 5-min saponification effectively removed these ester groups, leading to a 1.08-, 2.75-, and 2.50-fold increase in 48-h cellulose hydrolysis for 2-, 5-, and 8-h DES-pretreated samples, respectively. Analysis of chemical composition, surface morphology, crystallinity, and degree of polymerization revealed that these factors did not contribute to the enhanced hydrolysis observed in the saponified samples. This study concludes that saponification-induced de-esterification is the key factor driving the significant increase in enzymatic cellulose hydrolysis, highlighting the detrimental impact of esterification occurring during carboxylic acid-based DES pretreatment on subsequent cellulose saccharification.

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研究用羧酸类深共晶溶剂预处理玉米秸秆的酶法糖化过程中酯化诱导的抑制作用

氯化胆碱-乳酸是一种常见的基于羧酸的深共晶溶剂（DES），用于预处理玉米秸秆（CS）。尽管木质素和木聚糖被大量去除，但酶纤维素转化率仍低于 30%，仅略高于未经处理的 CS。结构表征证实，在预处理过程中，CS 的羟基与乳酸之间发生了酯化反应。短暂的 5 分钟皂化可有效去除这些酯基，从而使经过 2、5 和 8 小时 DES 预处理的样品在 48 小时内的纤维素水解率分别提高了 1.08、2.75 和 2.50 倍。对化学成分、表面形态、结晶度和聚合度的分析表明，皂化样品中观察到的水解作用增强与这些因素无关。本研究的结论是，皂化诱导的去酯化作用是促使酶促纤维素水解作用显著增加的关键因素，突出了在基于羧酸的 DES 预处理过程中发生的酯化作用对后续纤维素糖化作用的不利影响。

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来源期刊

Industrial Crops and Products 农林科学-农业工程

CiteScore

9.50

自引率

8.50%

发文量

1518

审稿时长

43 days

期刊介绍： Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.