Method development and application for multi-phenolic components in the processing of Dendrobium officinale using UPLC coupled with mass spectrometry and chemometric analysis

IF 5.6 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products Pub Date : 2024-11-09 DOI:10.1016/j.indcrop.2024.120009

Xinxin Yang , Pengfei Liu , Fengzhong Wang , Tingting Zhang , Nengbo Li , Cong Lu , Litao Tong , Jiameng Liu , Jing Sun , Bei Fan

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引用次数: 0

Abstract

Dendrobium officinale (DO) is a perennial herb that has been utilized medicinal and edible purposes for thousands of years, often transforming into 'Fengdou' after drying. Despite its long-standing use, the specific phenolic components present during DO processing have remained elusive. This study established a method for the simultaneous and accurate quantification of 27 phenolic compounds during DO processing employing ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), with 8 reference-free components optimized through an online strategy. Furthermore, correlation analysis and molecular docking were conducted to further screened and validated 6 phenolics with α-glucosidase inhibitory activity, including vanillin, p-coumaric acid, 4-hydroxybenzaldehyde, resveratrol, coniferyl alcohol, and dihydropinosylvin. Notably, resveratrol exhibited the lowest binding energy at −7.7 kcal/mol among these compounds. These findings significantly contribute to the development of the deep-processing techniques for DO and the hypoglycaemic functional food industry by providing data that support the elucidation of medicinal substances in DO.

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利用 UPLC 联用质谱法和化学计量分析法开发和应用铁皮石斛加工过程中多酚类成分的方法

铁皮石斛（Dendrobium officinale，DO）是一种多年生草本植物，数千年来一直被用于药用和食用目的，干燥后通常会变成 "凤斗"。尽管铁皮石斛的使用历史悠久，但其加工过程中的特定酚类成分却一直难以确定。本研究采用超高效液相色谱-串联质谱法（UPLC-MS/MS）建立了一种同时准确定量 DO 加工过程中 27 种酚类化合物的方法，并通过在线策略优化了 8 种无参考成分。此外，还进行了相关分析和分子对接，进一步筛选和验证了 6 种具有α-葡萄糖苷酶抑制活性的酚类物质，包括香兰素、对香豆酸、4-羟基苯甲醛、白藜芦醇、针叶醇和二氢白藜芦醇。值得注意的是，在这些化合物中，白藜芦醇的结合能最低，为-7.7 kcal/mol。这些发现为阐明 DO 中的药用物质提供了数据支持，从而大大促进了 DO 深加工技术的发展和降血糖功能食品行业的发展。

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来源期刊

Industrial Crops and Products 农林科学-农业工程

CiteScore

9.50

自引率

8.50%

发文量

1518

审稿时长

43 days

期刊介绍： Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.