Synthesis, characterisation, and anti-tumour activity of nano-immuno-conjugates for enhanced photodynamic therapy of oesophageal cancer stem cells

IF 6.9 2区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

Biomedicine & Pharmacotherapy Pub Date : 2024-11-16 DOI:10.1016/j.biopha.2024.117693

Onyisi Christiana Didamson , Rahul Chandran , Heidi Abrahamse

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引用次数: 0

Abstract

In recent times, oesophageal cancer has been listed as the eleventh most prevalent type of cancer. It is a lethal disease attributed to a high mortality rate, tumour metastasis and poor treatment outcome. A subset of oesophageal cancer referred to as stem cells (CSCs) has been revealed to drive carcinogenesis, metastasis, and treatment failure. Therefore, it is essential to target these CSCs to improve the efficacy of treatment for oesophageal cancer. In this present study, we employed a strategy to target oesophageal CSCs with a nano-immuno-conjugate (NIC) consisting of AlPcS₄Cl, gold nanoparticle (AuNPs) and anti-CD271 antibody synthesised using a chemical reaction. The synthesised NIC was characterised using ultraviolet-visible spectroscopy, transmission electron microscope (TEM), Fourier transform infra-red (FTIR) spectroscopy, dynamic light scattering (DLS), and zeta potential (ZP). The in vitro anti-tumour action of NIC-mediated photodynamic therapy (PDT) was performed on oesophageal CSCs using cell viability/cytotoxicity assays and morphological examination via light microscopy. The characterisation analysis confirmed the successful synthesis of the NIC. The synthesised nano-immuno-conjugates showed significant cytotoxicity and reduction in cell viability in the HKESC-1 oesophageal CSCs. Fluorescence microscopy confirmed the rapid internalisation of the targeted NIC in key cellular organelles of the CSCs, resulting in enhanced effects. Interestingly, NIC exhibited cytocompatibility with non-tumour WS1 cells, thus supporting its clinical application as a safe anti-tumour agent for enhanced PDT. The study demonstrates the improved effects of NIC-mediated PDT as targeted therapeutics against oesophageal CSCs.

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用于增强食道癌干细胞光动力疗法的纳米免疫共轭物的合成、表征和抗肿瘤活性。

近来，食道癌已被列为发病率第十一位的癌症类型。食道癌是一种致命疾病，死亡率高、肿瘤转移和治疗效果差。研究发现，食管癌的一个亚群被称为干细胞（CSCs），它们是癌变、转移和治疗失败的驱动因素。因此，针对这些干细胞提高食道癌的治疗效果至关重要。在本研究中，我们采用了一种靶向食管癌 CSCs 的策略，即利用化学反应合成由 AlPcS4Cl、金纳米粒子（AuNPs）和抗 CD271 抗体组成的纳米免疫共轭物（NIC）。利用紫外可见光谱、透射电子显微镜（TEM）、傅立叶变换红外光谱（FTIR）、动态光散射（DLS）和 zeta 电位（ZP）对合成的 NIC 进行了表征。利用细胞存活率/毒性测定法和光镜形态学检查法，对食道癌细胞间充质干细胞进行了 NIC 介导的光动力疗法（PDT）体外抗肿瘤作用研究。表征分析证实成功合成了 NIC。合成的纳米免疫共轭物在 HKESC-1 食管干细胞中显示出显著的细胞毒性并降低了细胞活力。荧光显微镜证实，靶向 NIC 在 CSCs 的关键细胞器中快速内化，从而增强了作用。有趣的是，NIC 与非肿瘤 WS1 细胞表现出细胞相容性，因此支持其作为一种安全的抗肿瘤药物应用于临床，以增强 PDT 的效果。这项研究表明，NIC 介导的光导光疗作为针对食道癌细胞间充质干细胞的靶向疗法，具有更好的效果。

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来源期刊

Biomedicine & Pharmacotherapy 医学-药学

CiteScore

11.90

自引率

2.70%

发文量

1621

审稿时长

48 days

期刊介绍： Biomedicine & Pharmacotherapy stands as a multidisciplinary journal, presenting a spectrum of original research reports, reviews, and communications in the realms of clinical and basic medicine, as well as pharmacology. The journal spans various fields, including Cancer, Nutriceutics, Neurodegenerative, Cardiac, and Infectious Diseases.