A novel method to assess photobiomodulation in stimulating regenerative capacity and vascularization in zebrafish.

IF 3.8 3区 医学 Q2 CELL BIOLOGY
Wound Repair and Regeneration Pub Date : 2025-01-01 Epub Date: 2024-11-16 DOI:10.1111/wrr.13234
Afnan Sedky Adly, Jean-Christophe Egea, Mahmoud Sedky Adly, Ivan Panayotov, Aya Sedky Adly, Eve Malthiery, Frederic Cuisinier
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引用次数: 0

Abstract

Photobiomodulation (PBM) therapy is a continuously growing approach to stimulating healing and reducing inflammation and pain. However, its effects in the fields of regenerative medicine and tissue engineering are still under investigation. Studying PBM effects on the regenerative capacity of zebrafish can allow the application of novel clinical approaches where the impact of PBM will be cross-linked with the stem-cell therapeutic approaches. This study was done to establish an in-vivo experimental setup for studying the effects of laser and ultraviolet therapy on zebrafish caudal-fin regeneration and vascularization. Thirty zebrafish were randomly and equally allocated into three groups. The caudal-fins of all zebrafish were amputated under anaesthesia. In the first control group, the caudal-fin was only monitored until fully regenerated. In the second group, the amputated-fin was irradiated with ultraviolet. Finally, in the third group, the amputated-fin was irradiated with laser. Caudal-fin regeneration and vascularization were assessed at days 0, 3, 6, 9, 12, and 15 in all fish. In terms of regeneration, the results indicated that it is possible to discriminate the regenerative effect of laser with the experimental setup as laser therapy showed a statistically significant difference when compared to control-group. It was also found that regenerative stimulation of the group that received ultraviolet therapy showed significant difference when compared to the control group. In terms of vascularization, there was a statistically significant difference in all groups of the study, which may suggest that laser as well as ultraviolet have limited effects in terms of improving vascularization. This study presented a novel, simple and inexpensive method for the assessment of PBM effects on zebrafish. Laser and ultraviolet therapy appeared to act as regenerative stimulators for caudal-fin regeneration of zebrafish. However, laser therapy results were, to some extent, better than ultraviolet therapy. This novel in-vivo design of the experiment led to more rapid and reproducible results than in-vitro experiments.

评估光生物调制刺激斑马鱼再生能力和血管形成的新方法。
光生物调制疗法(PBM)是一种不断发展的刺激愈合、减轻炎症和疼痛的方法。然而,它在再生医学和组织工程领域的效果仍在研究之中。研究PBM对斑马鱼再生能力的影响,可以应用新的临床方法,将PBM的影响与干细胞治疗方法交叉联系起来。本研究建立了一个体内实验装置,用于研究激光和紫外线疗法对斑马鱼尾鳍再生和血管形成的影响。30 只斑马鱼被随机平均分配为三组。所有斑马鱼的尾鳍均在麻醉状态下截断。在第一对照组中,只监测尾鳍直至完全再生。在第二组中,用紫外线照射截肢的尾鳍。最后,在第三组中,用激光照射截肢鳍。在第 0、3、6、9、12 和 15 天对所有鱼的尾鳍再生和血管形成进行评估。结果表明,在再生方面,激光疗法与对照组相比有显著的统计学差异,因此可以通过实验设置来区分激光的再生效果。研究还发现,与对照组相比,接受紫外线治疗组的再生刺激效果有显著差异。在血管生成方面,所有研究组的差异都有统计学意义,这可能表明激光和紫外线在改善血管生成方面的效果有限。本研究提出了一种新颖、简单、廉价的方法来评估 PBM 对斑马鱼的影响。激光和紫外线疗法似乎能刺激斑马鱼尾鳍的再生。不过,激光疗法的效果在一定程度上优于紫外线疗法。与体外实验相比,这种新颖的体内实验设计能更快速地得出可重复的结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Wound Repair and Regeneration
Wound Repair and Regeneration 医学-皮肤病学
CiteScore
5.90
自引率
3.40%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Wound Repair and Regeneration provides extensive international coverage of cellular and molecular biology, connective tissue, and biological mediator studies in the field of tissue repair and regeneration and serves a diverse audience of surgeons, plastic surgeons, dermatologists, biochemists, cell biologists, and others. Wound Repair and Regeneration is the official journal of The Wound Healing Society, The European Tissue Repair Society, The Japanese Society for Wound Healing, and The Australian Wound Management Association.
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