Blood serum interleukins levels and their relationship with colon tissue prostanoids in experimental ulcerative colitis.

Q4 Medicine

Wiadomosci lekarskie Pub Date : 2024-01-01 DOI:10.36740/WLek/192139

Iryna Vasylieva, Oksana Nakonechna, Liudmyla Popova, Natalia Yarmysh, Anastasiia Bezrodna, Nataliia Pustova

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引用次数: 0

Abstract

Objective: Aim: To study IL-1β, IL-6, IL-8, IL-10 blood serum levels and their relationship with colon tissue prostanoids in experimental ulcerative colitis.

Patients and methods: Materials and Methods: Blood serum interleukins and colon tissue prostanoids were determined by enzyme immunosorbent assay on three groups of WAG population rats (1st control group - rectal saline injection; 2nd control group - 50% ethanol injection; experimental group - injection of 2,4-dinitrobenzene-sulfonic acid in 50% ethanol).

Results: Results: The increased levels of all interleukins both in 2nd control and experimental groups versus 1st control group were found. No dif f erences in IL-6, IL-8 levels between experimental and 2nd control groups were revealed. The relationships between interleukins and PGF2α, between interleukins and TXB2 in all groups, and the appearance of correlations between interleukins and PGE2, between interleukins and PGI2 in experimental group were observed. Negative correlations between IL-8 and COX-2 in 1st control, between IL-8 and COX-1 in both 2nd control and experimental groups were found. Negative correlation between 8-epi-PGF2α and IL-8, positive correlation between 8-epi-PGF2α and PGF2α in experimental group were revealed.

Conclusion: Conclusions: Results obtained testify the possible role of PGF2α and 8-epi-PGF2α in the development of ulcerative colitis and justify the importance of research on the link of 8-epi-PGF2α, IL-8, PGF2α for understanding the mechanisms of development and progression of this disease. Increasing blood serum IL-8 level may be used as marker of reducing colon tissue COX-1 content in both ethanol- and 2,4-dinitrobenzenesulfonic acid-induced ulcerative colites.

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实验性溃疡性结肠炎的血清白细胞介素水平及其与结肠组织前列腺素的关系

研究目的目的：研究实验性溃疡性结肠炎患者血清中IL-1β、IL-6、IL-8、IL-10水平及其与结肠组织前列腺素的关系：材料与方法：采用酶联免疫吸附法测定三组 WAG 大鼠血清白细胞介素和结肠组织前列腺素的含量（对照组 1：直肠生理盐水注射；对照组 2：50%乙醇注射；实验组 3：50%乙醇中 2,4-二硝基苯磺酸注射）：结果：结果：结果：发现第二对照组和实验组的所有白细胞介素水平均高于第一对照组。实验组和第二对照组的 IL-6、IL-8 水平无差异。所有组的白细胞介素与 PGF2α、白细胞介素与 TXB2 之间均存在相关性，实验组的白细胞介素与 PGE2、白细胞介素与 PGI2 之间存在相关性。在第一对照组中，IL-8 与 COX-2 呈负相关；在第二对照组和实验组中，IL-8 与 COX-1 呈负相关。实验组 8-epi-PGF2α 与 IL-8 呈负相关，8-epi-PGF2α 与 PGF2α 呈正相关：结论研究结果证明了 PGF2α 和 8-epi-PGF2α 在溃疡性结肠炎发病过程中可能发挥的作用，并证明了研究 8-epi-PGF2α、IL-8、PGF2α 与溃疡性结肠炎发病和进展机制之间联系的重要性。在乙醇和 2,4-二硝基苯磺酸诱导的溃疡性结肠中，血清 IL-8 水平的升高可作为结肠组织 COX-1 含量降低的标志。

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来源期刊

Wiadomosci lekarskie Medicine-Medicine (all)

CiteScore

0.80

自引率

0.00%

发文量

482