The diagnostic value of peripheral blood lymphocyte testing in children with infectious mononucleosis.

IF 2 3区医学 Q2 PEDIATRICS

BMC Pediatrics Pub Date : 2024-11-16 DOI:10.1186/s12887-024-05228-6

Jingxin Zhou, Jia Zhang, Dan Zhu, Wentong Ma, Qing Zhong, Qin Shen, Jing Su

{"title":"The diagnostic value of peripheral blood lymphocyte testing in children with infectious mononucleosis.","authors":"Jingxin Zhou, Jia Zhang, Dan Zhu, Wentong Ma, Qing Zhong, Qin Shen, Jing Su","doi":"10.1186/s12887-024-05228-6","DOIUrl":null,"url":null,"abstract":"Objective: To investigate the diagnostic value of peripheral blood lymphocyte testing in children with infectious mononucleosis (IM).Methods: A total of 135 children with IM as the IM group and 100 healthy volunteers as the healthy group were included in this retrospective study. Peripheral blood lymphocyte subsets marked as CD3+, CD4+, CD8+, CD16 + CD56+, and CD19 + in the peripheral blood were quantified using flow cytometry. Statistical analysis was performed using the chi-square test, Kruskal-Wallis test, AUROC curve, and Kappa consistency test to assess the diagnostic value of these markers in IM.Results: The AUROC curve for CD8 + cells and for CD4+/CD8 + ratios both achieved a value of 1 with the sensitivity and specificity of 100% (P<0.001). The Kappa coefficients were 1 for CD8+, CD4+/CD8 + ratios and the combined EBV analysis, indicating a 100% consistency with the clinical diagnosis. Significant differences were also observed in the CD3+, CD4+, CD16 + CD56+, and CD19 + lymphocyte subsets between the IM group and the healthy group (P<0.05).Conclusion: The evaluation of CD8 + and CD4+/CD8 + ratios in peripheral blood lymphocytes represents a significant advancement in the diagnosis of IM. Peripheral blood lymphocyte testing offers a reliable, sensitive, and specific diagnostic tool to enhance the clinical management of children with IM.","PeriodicalId":9144,"journal":{"name":"BMC Pediatrics","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Pediatrics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12887-024-05228-6","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PEDIATRICS","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: To investigate the diagnostic value of peripheral blood lymphocyte testing in children with infectious mononucleosis (IM).

Methods: A total of 135 children with IM as the IM group and 100 healthy volunteers as the healthy group were included in this retrospective study. Peripheral blood lymphocyte subsets marked as CD3+, CD4+, CD8+, CD16 + CD56+, and CD19 + in the peripheral blood were quantified using flow cytometry. Statistical analysis was performed using the chi-square test, Kruskal-Wallis test, AUROC curve, and Kappa consistency test to assess the diagnostic value of these markers in IM.

Results: The AUROC curve for CD8 + cells and for CD4+/CD8 + ratios both achieved a value of 1 with the sensitivity and specificity of 100% (P<0.001). The Kappa coefficients were 1 for CD8+, CD4+/CD8 + ratios and the combined EBV analysis, indicating a 100% consistency with the clinical diagnosis. Significant differences were also observed in the CD3+, CD4+, CD16 + CD56+, and CD19 + lymphocyte subsets between the IM group and the healthy group (P<0.05).

Conclusion: The evaluation of CD8 + and CD4+/CD8 + ratios in peripheral blood lymphocytes represents a significant advancement in the diagnosis of IM. Peripheral blood lymphocyte testing offers a reliable, sensitive, and specific diagnostic tool to enhance the clinical management of children with IM.

查看原文本刊更多论文

传染性单核细胞增多症患儿外周血淋巴细胞检测的诊断价值。

目的研究传染性单核细胞增多症（IM）患儿外周血淋巴细胞检测的诊断价值：这项回顾性研究共纳入 135 名感染性单核细胞增多症儿童作为感染性单核细胞增多症组，100 名健康志愿者作为健康组。使用流式细胞术对外周血中标记为 CD3+、CD4+、CD8+、CD16 + CD56+ 和 CD19 + 的淋巴细胞亚群进行量化。采用卡方检验、Kruskal-Wallis 检验、AUROC 曲线和 Kappa 一致性检验进行统计分析，以评估这些标记物在 IM 中的诊断价值：结果：CD8+细胞的AUROC曲线和CD4+/CD8+比率的AUROC曲线均为1，灵敏度和特异性均为100%（PC结论：CD8+细胞和CD4+/CD8+比率的AUROC曲线均为1，灵敏度和特异性均为100%）：评估外周血淋巴细胞中的 CD8 + 和 CD4+/CD8 + 比率是 IM 诊断中的一大进步。外周血淋巴细胞检测提供了一种可靠、灵敏和特异的诊断工具，可加强对IM患儿的临床管理。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

BMC Pediatrics PEDIATRICS-

CiteScore

3.70

自引率

4.20%

发文量

683

审稿时长

3-8 weeks

期刊介绍： BMC Pediatrics is an open access journal publishing peer-reviewed research articles in all aspects of health care in neonates, children and adolescents, as well as related molecular genetics, pathophysiology, and epidemiology.