Antibody isotyping and cytokine profiling in natural cases of Burkholderia mallei infection (glanders) in equines

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine Pub Date : 2024-11-16 DOI:10.1016/j.cyto.2024.156799

Pooja , Naintara Thapa , Radha Rani , Karuppusamy Shanmugasundaram , Punit Jhandai , Rakshita , Tarun Kumar Bhattacharya , Harisankar Singha

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引用次数: 0

Abstract

Objectives

Immunological aspects of B. mallei infection were rarely studied in natural cases of equines. The present study was conducted to determine IgG, IgM, IgA and IgG (T) titre against recombinant Hcp1, TssA and TssB proteins and PilA-Hcp1-TssN-BipD and BpaB-BpaC- BMAA0553 chimeras and cytokine responses in glanders affected equine serum.

Methods

The study was conducted on serum samples collected from 151 glanders positive equines which include horses (n = 134), mules (n = 16), and donkeys (n = 01). The IgM, IgG, IgA and IgG (T) titre were determined against recombinant antigens by indirect ELISA and interleukin(IL)-1β, IL-17, IL-6, tumor necrosis factor alpha (TNF-α), monocyte chemoattractant protein 1 (MCP-1) and interferon gamma (IFN-γ) responses were measured by commercial ELISA kits.

Results

The study showed that glanders affected equines elicited strong antibody response against Hcp1, moderate responses against TssA and TssB, and weak responses against two chimeras. Among the cytokines, IL-1β, MCP-1, IL-17 and IL-6 concentration were significantly higher in glanders affected equine serum.

Conclusion

We found that IgG antibody titre was higher than IgM, IgG (T) and IgA isotypes and Hcp1 was most predominant antibody inducers in comparison to TssA, TssB, PilA-Hcp1-TssN-BipD and BpaB-BpaC-T2SS proteins. The elevated level of IL-1β, MCP-1, IL-17, IL-6, IFN-γ and TNF-α observed in this study support the important role of this cytokines for augmenting cellular defence by recruitment of macrophages, neutrophil and dendritic cells against B. mallei infection. Further studies should be conducted to determine memory cell responses in natural cases of equine glanders using recombinant B. mallei proteins for identifying well-characterized immuno-protective vaccine candidates.

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马伯克霍尔德氏菌感染（传染性单核细胞增多症）自然病例的抗体分型和细胞因子分析。

目的：很少在马的自然病例中研究马雷杆菌感染的免疫学方面。本研究旨在确定受腺疽影响的马血清中针对重组 Hcp1、TssA 和 TssB 蛋白、PilA-Hcp1-TssN-BipD 和 BpaB-BpaC- BMAA0553 嵌合体的 IgG、IgM、IgA 和 IgG (T) 滴度以及细胞因子反应：研究对象为从 151 匹腺疽阳性马（包括马（n = 134）、骡（n = 16）和驴（n = 01））采集的血清样本。采用间接 ELISA 法测定重组抗原的 IgM、IgG、IgA 和 IgG (T) 滴度，采用商用 ELISA 试剂盒测定白细胞介素 (IL)-1β、IL-17、IL-6、肿瘤坏死因子α(TNF-α)、单核细胞趋化蛋白 1 (MCP-1) 和干扰素γ (IFN-γ) 反应：研究表明，传染性单核细胞增多症马对 Hcp1 产生强抗体反应，对 TssA 和 TssB 产生中度反应，对两种嵌合体产生弱反应。在细胞因子中，腺病马血清中 IL-1β、MCP-1、IL-17 和 IL-6 的浓度明显较高：结论：我们发现，IgG 抗体滴度高于 IgM、IgG (T) 和 IgA 同型，与 TssA、TssB、PilA-Hcp1-TssN-BipD 和 BpaB-BpaC-T2SS 蛋白相比，Hcp1 是最主要的抗体诱导物。本研究中观察到的 IL-1β、MCP-1、IL-17、IL-6、IFN-γ 和 TNF-α 水平的升高证明了这些细胞因子在通过招募巨噬细胞、中性粒细胞和树突状细胞来增强细胞防御功能以抵抗 B. mallei 感染方面的重要作用。应开展进一步研究，利用重组 B. mallei 蛋白确定马传染性单核细胞增多症自然病例中的记忆细胞反应，以确定特征明确的免疫保护性候选疫苗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytokine 医学-免疫学

CiteScore

7.60

自引率

2.60%

发文量

262

审稿时长

48 days

期刊介绍： The journal Cytokine has an open access mirror journal Cytokine: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. * Devoted exclusively to the study of the molecular biology, genetics, biochemistry, immunology, genome-wide association studies, pathobiology, diagnostic and clinical applications of all known interleukins, hematopoietic factors, growth factors, cytotoxins, interferons, new cytokines, and chemokines, Cytokine provides comprehensive coverage of cytokines and their mechanisms of actions, 12 times a year by publishing original high quality refereed scientific papers from prominent investigators in both the academic and industrial sectors. We will publish 3 major types of manuscripts: 1) Original manuscripts describing research results. 2) Basic and clinical reviews describing cytokine actions and regulation. 3) Short commentaries/perspectives on recently published aspects of cytokines, pathogenesis and clinical results.