Effect of oxyresveratrol under in vitro lipopolysaccharide-induced periodontitis environment.

IF 2.6 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

BMC Oral Health Pub Date : 2024-11-15 DOI:10.1186/s12903-024-05128-2

Ju Ri Ye, Seung Hwan Park, Sang Wook Kang, Kyu Hwan Kwack, Yong Kwon Chae, Hyo-Seol Lee, Sung Chul Choi, Ok Hyung Nam

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引用次数: 0

Abstract

Background: Oxyresveratrol is the main constituent of mulberries and has many bioactive properties beneficial to human health. The purpose of this study was to assess the anti-inflammatory effects of oxyresveratrol on in vitro periodontitis model.

Methods: Human periodontal ligament cells were treated with oxyresveratrol (0, 10, and 20 µg/mL) for 72 h. Cell viability and flow cytometry assays were performed. To investigate anti-inflammatory effect of oxyresveratrol on periodontal inflammation, nitric oxide production under lipopolysaccharide stimulation was assessed. Next, expression of biomarkers associated periodontal inflammation was evaluated. Scratch wound assay was performed to evaluate cell migration/proliferation potential of oxyresveratrol under lipopolysaccharide stimulation.

Results: Periodontal ligament cell toxicity was not observed in oxyresveratrol treatment. Oxyresveratrol treatment significantly inhibited nitric oxide production and reduced MMP-2, MMP-9, TNF-α, IL-6, and IL-8 expressions after lipopolysaccharide stimulation. Regarding cell migration/proliferation, open wound area in oxyresveratrol (33.28 ± 6.80%) was the lowest (p < 0.05).

Conclusions: Within the limits of this study, oxyresveratrol inhibited lipopolysaccharide-induced inflammation in periodontal ligament cells and promoted periodontal ligament cell migration/proliferation. These findings suggest that oxyresveratrol could be valuable for the management of periodontal diseases.

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氧白藜芦醇在体外脂多糖诱导的牙周炎环境中的作用。

背景：氧白藜芦醇是桑葚的主要成分，具有许多有益于人类健康的生物活性特性。本研究的目的是评估氧白藜芦醇对体外牙周炎模型的抗炎作用。方法：用氧白藜芦醇（0、10 和 20 µg/mL）处理人类牙周韧带细胞 72 小时。为了研究氧白藜芦醇对牙周炎症的抗炎作用，评估了脂多糖刺激下一氧化氮的产生。接着，评估了与牙周炎症相关的生物标志物的表达。此外，还进行了划痕伤口试验，以评估氧白藜芦醇在脂多糖刺激下的细胞迁移/增殖潜力：结果：氧白藜芦醇治疗未观察到牙周韧带细胞毒性。氧白藜芦醇能显著抑制一氧化氮的产生，并降低脂多糖刺激后MMP-2、MMP-9、TNF-α、IL-6和IL-8的表达。在细胞迁移/增殖方面，氧白藜芦醇的开放性伤口面积（33.28 ± 6.80%）最小（p 结论）：在本研究的范围内，氧白藜芦醇可抑制脂多糖诱导的牙周韧带细胞炎症，并促进牙周韧带细胞迁移/增殖。这些研究结果表明，氧白藜芦醇对牙周疾病的治疗具有重要价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Oral Health DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.90

自引率

6.90%

发文量

481

审稿时长

6-12 weeks

期刊介绍： BMC Oral Health is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of disorders of the mouth, teeth and gums, as well as related molecular genetics, pathophysiology, and epidemiology.