Phenotype, physiology and transcriptome analysis of vivipary in cucumber

IF 3.9 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2024-11-12 DOI:10.1016/j.scienta.2024.113790

Jiaxi Li, Xiaohui Tian, Zhiwei Qin, Mei Gao, Xinfang Jiang, Ming Xin

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引用次数: 0

Abstract

Vivipary is a common phenomenon in nature. However, vivipary in cucumber affects seed production and processing, and it is necessary to understand the mechanism. In this study, the phenotype, physiology and transcriptome of easy-viviparous cucumber inbred line ‘D1158’ were analyzed. The vivipary manifested on the 35th day after pollination, and the viviparous germination rate reached 50 % on the 55th day after pollination. The viviparous germplasm resources were mainly concentrated in South China and North China types rather than European greenhouse and pickle types. Besides, high temperature, withered plants, and postripeness promoted vivipary. Differentially expressed genes in transcriptome were enriched in hormone signal transduction, energy and nutrient metabolism, and cellular component biosynthesis pathways. BR, CTK, IAA, and JA signals were activated, while ABA and ETH signals were inhibited in vivipary. Starch degradation and biosynthesis of soluble sugars, amino acids, fatty acids, and phenylpropanoids were up-regulated, possibly facilitating cell proliferation in vivipary. A potential regulatory pattern in the formation of vivipary in cucumber was constructed, and candidate genes were predicted. The results may enhance the comprehension of vivipary in cucumber and provide a basis for future research.

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黄瓜胎生的表型、生理和转录组分析

胎生是自然界的一种常见现象。然而，黄瓜的胎生现象会影响种子的生产和加工，因此有必要了解其机理。本研究分析了易胎生黄瓜近交系'D1158'的表型、生理和转录组。结果表明，黄瓜在授粉后第35天出现胎生，授粉后第55天胎生发芽率达到50%。胎生种质资源主要集中在华南和华北类型，而非欧洲温室和泡菜类型。此外，高温、植株枯萎和后熟也促进了胎生。转录组中的差异表达基因主要集中在激素信号转导、能量和养分代谢以及细胞组分生物合成途径。在胎生期，BR、CTK、IAA 和 JA 信号被激活，而 ABA 和 ETH 信号被抑制。淀粉降解以及可溶性糖、氨基酸、脂肪酸和苯丙酮的生物合成受到上调，这可能促进了胎生期细胞的增殖。研究构建了黄瓜胎生期形成的潜在调控模式，并预测了候选基因。这些结果可加深对黄瓜胎生的理解，并为今后的研究提供依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.