Unveiling the regulatory mechanism of pulsed electric field modified instant soy milk powder: Exploring allergenicity, conformation, and epitopes

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2024-11-15 DOI:10.1016/j.foodchem.2024.142071

Jie Ding, Yaru Li, Limin Zhong, Xinqing Lu, Zhijie Bao, Songyi Lin

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Abstract

Glycinin and β-conglycinin are prominent allergens, limiting the safety of soy and soy products in a wide range of applications. The study investigated the effects of pulsed electric field (PEF) on allergenicity, conformation, and epitopes of instant soy milk powder (ISMP) and the simulated digestion products (SDP), and to preliminarily elucidate the regulatory mechanism using multispectroscopy and peptidomics. The allergenicity of SDP and ISMP treated at 20 and 30 kV/cm were reduced and conformations were altered, especially decrease or disappearance of α-helix, enhancement of random coil, and reduction of hydrogen and disulfide bonds in SDP (P < 0.05). Furthermore, their digestion-resistant linear epitopes (DRLE) were differed, such as RSQSDN, EEEEQRQQ, and SRNPIY, therein N and P might be critical amino acids affecting their allergenicity. Overall, PEF reduced the ISPM allergenicity by altering conformations, increasing sensitivities to gastrointestinal digestive enzymes, and modulating binding sites to IgE by covering or disrupting linear epitopes.

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揭示脉冲电场改性速溶豆奶粉的调控机制：探索过敏性、构象和表位点

甘氨素和β-共甘氨素是突出的过敏原，限制了大豆和大豆制品在广泛应用中的安全性。本研究探讨了脉冲电场（PEF）对速溶豆奶粉（ISMP）和模拟消化产物（SDP）的过敏性、构象和表位的影响，并利用多光谱学和肽组学初步阐明了其调控机制。经 20 和 30 kV/cm 处理的 SDP 和 ISMP 的致敏性降低，构象发生改变，尤其是 SDP 中的α-螺旋减少或消失，无规线圈增强，氢键和二硫键减少（P < 0.05）。此外，它们的抗消化线性表位（DRLE）也存在差异，如 RSQSDN、EEEEQRQQ 和 SRNPIY，其中 N 和 P 可能是影响其过敏性的关键氨基酸。总之，PEF 通过改变构象、增加对胃肠道消化酶的敏感性以及通过覆盖或破坏线性表位来调节与 IgE 的结合位点，从而降低了 ISPM 的过敏性。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.