Genetic transformation in conifers: current status and future prospects.

Forestry research Pub Date : 2024-03-21 eCollection Date: 2024-01-01 DOI:10.48130/forres-0024-0007
Huanhuan Zhao, Jinfeng Zhang, Jian Zhao, Shihui Niu
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Abstract

Genetic transformation has been a cornerstone in plant molecular biology research and molecular design breeding, facilitating innovative approaches for the genetic improvement of trees with long breeding cycles. Despite the profound ecological and economic significance of conifers in global forestry, the application of genetic transformation in this group has been fraught with challenges. Nevertheless, genetic transformation has achieved notable advances in certain conifer species, while these advances are confined to specific genotypes, they offer valuable insights for technological breakthroughs in other species. This review offers an in-depth examination of the progress achieved in the genetic transformation of conifers. This discussion encompasses various factors, including expression vector construction, gene-delivery methods, and regeneration systems. Additionally, the hurdles encountered in the pursuit of a universal model for conifer transformation are discussed, along with the proposal of potential strategies for future developments. This comprehensive overview seeks to stimulate further research and innovation in this crucial field of forest biotechnology.

针叶树的遗传转化:现状与前景。
遗传转化一直是植物分子生物学研究和分子设计育种的基石,有助于采用创新方法对育种周期长的树木进行遗传改良。尽管针叶树在全球林业中具有深远的生态和经济意义,但基因转化在针叶树中的应用一直充满挑战。尽管如此,基因转化在某些针叶树种中取得了显著进展,虽然这些进展仅限于特定的基因型,但它们为其他树种的技术突破提供了宝贵的启示。本综述深入探讨了针叶树基因转化方面取得的进展。讨论涉及各种因素,包括表达载体的构建、基因传递方法和再生系统。此外,还讨论了在寻求针叶树基因转化通用模型过程中遇到的障碍,并提出了未来发展的潜在战略。本综述旨在促进森林生物技术这一关键领域的进一步研究和创新。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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