Recombinant feline herpesvirus-1 (FHV-1) expressing granulocyte colony-stimulating factor (G-CSF) exhibits enhanced protective efficacy in felines

IF 2.8 3区医学 Q3 VIROLOGY

Virology Pub Date : 2024-10-31 DOI:10.1016/j.virol.2024.110282

Mengfang Yang , Yuzhou Jiao , Yuanyuan Yan , Zhen Fu , Lisha Li , Zirui Liu , Lingying Fang , Xiaoshuai Hu , Benyuan Wu , Yuejun Shi , Mengxia Li , Zhou Shen , Guiqing Peng

{"title":"Recombinant feline herpesvirus-1 (FHV-1) expressing granulocyte colony-stimulating factor (G-CSF) exhibits enhanced protective efficacy in felines","authors":"Mengfang Yang , Yuzhou Jiao , Yuanyuan Yan , Zhen Fu , Lisha Li , Zirui Liu , Lingying Fang , Xiaoshuai Hu , Benyuan Wu , Yuejun Shi , Mengxia Li , Zhou Shen , Guiqing Peng","doi":"10.1016/j.virol.2024.110282","DOIUrl":null,"url":null,"abstract":"<div><div>Vaccine efficacy relies not only on antigens but also on immunomodulatory agents/adjuvants that are often used to stimulate the immune system and enhance the immune response. However, current immunomodulatory agents are used to increase the immune response induced by viral or bacterial inactivated vaccine antigens, bacterial toxoids or polysaccharides but not attenuated live viruses. Based on the immunomodulatory functions of G-CSF and the characteristics of feline herpesvirus-1 (FHV-1) as an expression vector, a recombinant virus expressing feline G-CSF (WH2020-ΔTK/gI/gE-G-CSF) was constructed. The growth dynamics of WH2020-ΔTK/gI/gE-G-CSF were similar to those of WH2020-ΔTK/gI/gE. Compared with kittens vaccinated with WH2020 Δ TK/gI/gE, felines inoculated with WH2020 ΔTK/gI/gE-G-CSF produced more neutralizing antibodies and neutrophils, further alleviating clinical symptoms after FHV-1 infection. Taken together, our results revealed the potential of G-CSF as an ideal immune potentiator that can augment immune responses to FHV-1 and even other attenuated live vaccines.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"601 ","pages":"Article 110282"},"PeriodicalIF":2.8000,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Virology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0042682224003064","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VIROLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Vaccine efficacy relies not only on antigens but also on immunomodulatory agents/adjuvants that are often used to stimulate the immune system and enhance the immune response. However, current immunomodulatory agents are used to increase the immune response induced by viral or bacterial inactivated vaccine antigens, bacterial toxoids or polysaccharides but not attenuated live viruses. Based on the immunomodulatory functions of G-CSF and the characteristics of feline herpesvirus-1 (FHV-1) as an expression vector, a recombinant virus expressing feline G-CSF (WH2020-ΔTK/gI/gE-G-CSF) was constructed. The growth dynamics of WH2020-ΔTK/gI/gE-G-CSF were similar to those of WH2020-ΔTK/gI/gE. Compared with kittens vaccinated with WH2020 Δ TK/gI/gE, felines inoculated with WH2020 ΔTK/gI/gE-G-CSF produced more neutralizing antibodies and neutrophils, further alleviating clinical symptoms after FHV-1 infection. Taken together, our results revealed the potential of G-CSF as an ideal immune potentiator that can augment immune responses to FHV-1 and even other attenuated live vaccines.

查看原文本刊更多论文

表达粒细胞集落刺激因子（G-CSF）的重组猫疱疹病毒-1（FHV-1）在猫科动物中表现出更强的保护效力。

疫苗的疗效不仅取决于抗原，还取决于免疫调节剂/佐剂，它们通常用于刺激免疫系统和增强免疫反应。然而，目前的免疫调节剂用于提高病毒或细菌灭活疫苗抗原、细菌毒素或多糖诱导的免疫反应，而不是减毒活疫苗。根据 G-CSF 的免疫调节功能和猫疱疹病毒-1（FHV-1）作为表达载体的特点，构建了表达猫 G-CSF 的重组病毒（WH2020-ΔTK/gI/gE-G-CSF）。WH2020-ΔTK/gI/gE-G-CSF的生长动态与WH2020-ΔTK/gI/gE相似。与接种WH2020 ΔTK/gI/gE的幼猫相比，接种WH2020 ΔTK/gI/gE-G-CSF的猫产生了更多的中和抗体和中性粒细胞，进一步减轻了FHV-1感染后的临床症状。综上所述，我们的研究结果揭示了 G-CSF 作为一种理想的免疫增强剂的潜力，它可以增强对 FHV-1 甚至其他减毒活疫苗的免疫反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Virology 医学-病毒学

CiteScore

6.00

自引率

0.00%

发文量

157

审稿时长

50 days

期刊介绍： The journal features articles on virus replication, virus-host biology, viral pathogenesis, immunity to viruses, virus structure, and virus evolution and ecology. We aim to publish papers that provide advances to the understanding of virus biology.