Prevalence and distribution of serological characteristics of weak ABO subgroups in the Chinese population

IF 1.8 Q3 HEMATOLOGY

Hematology, Transfusion and Cell Therapy Pub Date : 2024-11-01 DOI:10.1016/j.htct.2024.04.127

Zhongying Wang , Sha Jin , Jiewei Zheng , Chenrui Qian , Xiaohong Caib , Dong Xiang

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Abstract

Objectives

This study aimed to determine the true frequency of weak ABO subgroups and to investigate the serological characteristics of various subgroup alleles in the Chinese population.

Methods

A total of 2,945,643 blood samples were collected from January 2009 to December 2017. After initial screening and re-examination using automatic blood group analyzers, all ABO-discrepant samples were confirmed by standard serological analysis and molecular detection by DNA sequencing. The true frequency of weak ABO subgroups was determined by the number of ABO subgroup donors and the missed detection rate. The ABO antigen expressions corresponding to subgroup alleles were analyzed by the agglutination intensity of red blood cells.

Results

The detection rate of ABO subgroups was 0.031 % (927/2,945,643). Considering the missed detection rate (27.81 %), the true frequency of ABO subgroups in the Chinese population was 0.044 %. The three most common genetic variations among blood donors in Shanghai were BA.04, BW.12 and BA.02. BW.03 showed the weakest B antigen expression (6.00 ± 1.97) and B^var-¹ the strongest (9.20 ± 1.10).

Conclusion

Many ABO subgroups were missed. BA.04 was found to be the most common subgroup allele in the Chinese population. Different ABO subgroup alleles exhibit different ABO antigen expression patterns.

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中国人群中 ABO 弱亚群血清学特征的流行率和分布。

研究目的本研究旨在确定中国人群中 ABO 弱亚群的真实频率，并调查各种亚群等位基因的血清学特征：方法：从 2009 年 1 月至 2017 年 12 月，共采集了 2,945,643 份血样。在使用自动血型分析仪进行初筛和复检后，所有ABO差异样本均通过标准血清学分析和DNA测序的分子检测得到确认。ABO 弱亚型的真实频率由 ABO 亚型献血者人数和漏检率决定。通过红细胞凝集强度分析亚群等位基因对应的 ABO 抗原表达：结果：ABO 亚群的检出率为 0.031 %（927/2,945,643）。考虑到漏检率（27.81%），中国人群中 ABO 亚型的真实频率为 0.044%。上海献血者中最常见的三种基因变异是 BA.04、BW.12 和 BA.02。BW.03的B抗原表达最弱（6.00 ± 1.97），Bvar-1最强（9.20 ± 1.10）：结论：许多 ABO 亚群被遗漏。结论：许多 ABO 亚群被遗漏，BA.04 是中国人群中最常见的亚群等位基因。不同的 ABO 亚群等位基因表现出不同的 ABO 抗原表达模式。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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