Involvement of UDP-glycosyltransferase of Kalanchoe pinnata (Lam.) Pers in quercitrin biosynthesis and consequent antioxidant defense

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-11-05 DOI:10.1016/j.plaphy.2024.109274

Chenglang Pan , Xianfeng Wang , Yunjie Xie , Chonglong Wei , Xiaohong Sun , Shenshen Zhang , Xiangzhen Yu , Xiaofeng Chen , Xi Chen , Caijin Tuo , Jiazeng Pan , Zhizhen Fang , Jianming Chen

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引用次数: 0

Abstract

Plants in the Crassulaceae family are rich in flavonoids, which may contribute to their high antioxidant activity and strong tolerance to abiotic stresses. Kalanchoe spp. are known to have high antioxidant activity, but the main components responsible for this activity and the mechanisms that control antioxidant accumulation in these plants are unknown. In this study, we identified the dominant flavonoid and investigated the mechanisms of its accumulation in Kalanchoe leaves. A wide metabolomics analysis showed that flavonoids were the most enriched metabolites in the leaves of Kalanchoe pinnata (Lam.) Pers, Kalanchoe delagoensis (Eckl. et Zeyh.) Druce, and Kalanchoe fedtschenkoi ‘Rosy Dawn’. Among these metabolites, levels of quercitrin were significantly higher in K. pinnata (Lam.) Pers than in the other two species. RNA-seq and targeted metabolomics analyses conducted on four different K. pinnata (Lam.) Pers tissues indicated that UDP-glycosyltransferase regulates the synthesis of quercitrin. Using in vitro enzyme assays and overexpression in tobacco we demonstrated that KpUGT74F catalyzes glycosylation of quercetin to produce quercitrin. This study identified the major antioxidant substances and their key regulatory mechanisms in Kalanchoe pinnata (Lam.) Pers to provide an important theoretical basis for understanding the high stress tolerance of these plants.

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Kalanchoe pinnata (Lam.) Pers 的 UDP-糖基转移酶参与槲皮素的生物合成和抗氧化防御。

菊科植物富含黄酮类化合物，这可能是它们具有高抗氧化活性和对非生物胁迫具有较强耐受力的原因。众所周知，Kalanchoe 属植物具有很高的抗氧化活性，但导致这种活性的主要成分以及控制这些植物中抗氧化剂积累的机制尚不清楚。在这项研究中，我们确定了主要的黄酮类化合物，并研究了其在 Kalanchoe 叶片中的积累机制。广泛的代谢组学分析表明，类黄酮是 Kalanchoe pinnata (Lam.) Pers、Kalanchoe delagoensis (Eckl. et Zeyh.) Druce 和 Kalanchoe fedtschenkoi 'Rosy Dawn'叶片中含量最高的代谢物。在这些代谢物中，K. pinnata (Lam.) Pers 的槲皮素含量明显高于其他两个物种。对四种不同的 K. pinnata (Lam.) Pers 组织进行的 RNA-seq 和靶向代谢组学分析表明，UDP-糖基转移酶调节槲皮素的合成。通过体外酶测定和在烟草中的过表达，我们证明了 KpUGT74F 催化槲皮素的糖基化以产生槲皮素。这项研究确定了 Kalanchoe pinnata (Lam.) Pers 的主要抗氧化物质及其关键调控机制，为了解这些植物的高抗逆性提供了重要的理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.