Long-term optical imaging of the spinal cord in awake behaving mice

IF 36.1 1区 生物学 Q1 BIOCHEMICAL RESEARCH METHODS
Biafra Ahanonu, Andrew Crowther, Artur Kania, Mariela Rosa-Casillas, Allan I. Basbaum
{"title":"Long-term optical imaging of the spinal cord in awake behaving mice","authors":"Biafra Ahanonu, Andrew Crowther, Artur Kania, Mariela Rosa-Casillas, Allan I. Basbaum","doi":"10.1038/s41592-024-02476-3","DOIUrl":null,"url":null,"abstract":"Advances in optical imaging and fluorescent biosensors enable study of the spatiotemporal and long-term neural dynamics in the brain of awake animals. However, methodological difficulties and fibrosis limit similar advances in the spinal cord. Here, to overcome these obstacles, we combined in vivo application of fluoropolymer membranes that inhibit fibrosis, a redesigned implantable spinal imaging chamber and improved motion correction methods that together permit imaging of the spinal cord in awake behaving mice, for months to over a year. We demonstrated a robust ability to monitor axons, identified a spinal cord somatotopic map, performed months-long imaging in freely moving mice, conducted Ca2+ imaging of neural dynamics in behaving mice responding to pain-provoking stimuli and observed persistent microglial changes after nerve injury. The ability to couple in vivo imaging and behavior at the spinal cord level will drive insights not previously possible at a key location for somatosensory transmission to the brain. Long-term imaging in the spinal cord is achieved by placing a fluoropolymer membrane on the spinal cord, which reduces fibrosis. This approach, combined with deep-learning-based motion correction, enables months-long imaging of the same neurons.","PeriodicalId":18981,"journal":{"name":"Nature Methods","volume":"21 12","pages":"2363-2375"},"PeriodicalIF":36.1000,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature Methods","FirstCategoryId":"99","ListUrlMain":"https://www.nature.com/articles/s41592-024-02476-3","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

Advances in optical imaging and fluorescent biosensors enable study of the spatiotemporal and long-term neural dynamics in the brain of awake animals. However, methodological difficulties and fibrosis limit similar advances in the spinal cord. Here, to overcome these obstacles, we combined in vivo application of fluoropolymer membranes that inhibit fibrosis, a redesigned implantable spinal imaging chamber and improved motion correction methods that together permit imaging of the spinal cord in awake behaving mice, for months to over a year. We demonstrated a robust ability to monitor axons, identified a spinal cord somatotopic map, performed months-long imaging in freely moving mice, conducted Ca2+ imaging of neural dynamics in behaving mice responding to pain-provoking stimuli and observed persistent microglial changes after nerve injury. The ability to couple in vivo imaging and behavior at the spinal cord level will drive insights not previously possible at a key location for somatosensory transmission to the brain. Long-term imaging in the spinal cord is achieved by placing a fluoropolymer membrane on the spinal cord, which reduces fibrosis. This approach, combined with deep-learning-based motion correction, enables months-long imaging of the same neurons.

Abstract Image

清醒行为小鼠脊髓的长期光学成像。
光学成像和荧光生物传感器的进步使研究清醒动物大脑的时空和长期神经动态成为可能。然而,方法上的困难和脊髓纤维化限制了脊髓的类似研究进展。在这里,为了克服这些障碍,我们将抑制纤维化的含氟聚合物膜、重新设计的植入式脊髓成像室和改进的运动校正方法结合在一起,使脊髓成像在清醒的行为小鼠体内可持续数月至一年以上。我们展示了监测轴突的强大能力,确定了脊髓体位图,对自由活动的小鼠进行了长达数月的成像,对行为小鼠对疼痛刺激做出反应时的神经动态进行了 Ca2+ 成像,并观察到神经损伤后小胶质细胞的持续变化。将脊髓水平的活体成像和行为结合起来的能力将促使人们深入了解躯体感觉传递到大脑的关键位置,而这在以前是不可能实现的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Nature Methods
Nature Methods 生物-生化研究方法
CiteScore
58.70
自引率
1.70%
发文量
326
审稿时长
1 months
期刊介绍: Nature Methods is a monthly journal that focuses on publishing innovative methods and substantial enhancements to fundamental life sciences research techniques. Geared towards a diverse, interdisciplinary readership of researchers in academia and industry engaged in laboratory work, the journal offers new tools for research and emphasizes the immediate practical significance of the featured work. It publishes primary research papers and reviews recent technical and methodological advancements, with a particular interest in primary methods papers relevant to the biological and biomedical sciences. This includes methods rooted in chemistry with practical applications for studying biological problems.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信