Pathogenicity and sub-lethal activity of orally administered entomopathogenic fungi against two adult mosquito species, Aedes aegypti (Diptera: Culicidae) and Anopheles stephensi (Diptera: Culicidae)

IF 3.6 3区生物学 Q1 ZOOLOGY

Journal of invertebrate pathology Pub Date : 2024-11-01 DOI:10.1016/j.jip.2024.108233

Sikandar Hussain , Hirotaka Kanuka , Aurelie Rakotondrafara , Masayuki Tani , Daigo Aiuchi

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Abstract

Entomopathogenic fungi (EPF) are known for their efficacy in controlling adult mosquito populations by penetrating through their cuticle. However, the effect of oral administration of EPF on the biological parameters of Aedes aegypti and Anopheles stephensi remains largely unexplored. This study aimed to assess the effect of orally administrated EPF isolates on the survival, feeding behavior, fecundity, fertility, follicle development and host-searching behavior in response to yeast-generated CO₂ of Ae. aegypti and An. stephensi. An initial screening of 50 isolates involved exposure of adult Ae. aegypti and An. stephensi by integument inoculation. Subsequently, the entomopathogenic effect of the five highly virulent isolates was confirmed through oral administration revealing Beauveria pseudobassiana 42–51 as a potent mosquito killer. B. pseudobassiana 42–51 was administered orally to evaluate sub-lethal effects. The results showed a 63 % and 43 % reduction in blood feeding of Ae. aegypti and An. stephensi, respectively. Furthermore, a decrease in egg hatching rate was observed, with a reduction of 83% for Ae. aegypti and 74% for An. stephensi on the seventh day following fungal administration, showing decreased hatchability in both species. Poor and abnormal follicle development was observed in both mosquito species. Also, the host-searching behavior was evaluated by attraction to CO₂ utilizing a Y-tube olfactometer. A tendency of reduction in the attraction rate towards the odor was observed three days post-fungal administration. These findings underscore the significant impact of oral administration of B. pseudobassiana 42–51 on mosquitoes, highlighting not only its lethal effects but also sub lethal impacts on their biology. Moreover, this fungus may exhibit the potential to simultaneously control both mosquito species and serve as a biocontrol agent for the management of vector-borne diseases.

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口服昆虫病原真菌对埃及伊蚊（Diptera: Culicidae）和按蚊（Diptera: Culicidae）两种成蚊的致病性和亚致死活性。

众所周知，昆虫病原真菌（EPF）可以穿透蚊子的角质层，有效控制成蚊数量。然而，口服 EPF 对埃及伊蚊和史蒂芬按蚊生物参数的影响在很大程度上仍未得到探讨。本研究旨在评估口服 EPF 分离物对埃及伊蚊和史蒂芬伊蚊的存活率、摄食行为、繁殖力、生育力、卵泡发育以及对酵母产生的 CO2 的宿主寻找行为的影响。在对 50 个分离物进行初步筛选时，埃及伊蚊和史蒂芬伊蚊成虫被接种到酵母菌中。随后，通过口服确认了五种高致病性分离物的昆虫致病作用，发现假蝙蝠蛾 42-51 是一种有效的蚊子杀手。通过口服 42-51 型假疣梭子蟹来评估亚致死效应。结果显示，埃及姬蚊和史蒂芬姬蚊的吸血量分别减少了 63% 和 43%。此外，还观察到卵孵化率下降，在给药后的第七天，埃及蚁的卵孵化率下降了 83%，而史蒂芬蚁的卵孵化率下降了 74%，这表明这两种害虫的孵化率都有所下降。在两种蚊子中都观察到卵泡发育不良和异常。此外，还利用 Y 型管嗅觉仪通过对二氧化碳的吸引来评估宿主的搜寻行为。在施用真菌三天后，观察到对气味的吸引率呈下降趋势。这些发现强调了口服假巴西蘑菇 42-51 对蚊子的重大影响，不仅突出了其致死效应，而且还对蚊子的生物学产生了亚致死影响。此外，这种真菌有可能同时控制两种蚊子，并作为一种生物控制剂用于病媒传播疾病的管理。

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来源期刊

Journal of invertebrate pathology 生物-动物学

CiteScore

6.10

自引率

5.90%

发文量

审稿时长

1 months

期刊介绍： The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates. The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.