Neutrophil extracellular traps in the cross-talk between periodontitis and chronic kidney disease.

IF 2.6 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

BMC Oral Health Pub Date : 2024-11-08 DOI:10.1186/s12903-024-05071-2

Shucheng Hu, Ruhan Yang, Wenying Yang, Jiaqi Tang, Weijun Yu, Dan Zhao, Lu Lin, Yuting Gu, Min Jin, Ziyuan Xu, Qin Wang, Eryi Lu

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引用次数: 0

Abstract

Background: The objective was to evaluate the level of neutrophil extracellular traps (NETs) in patients with chronic kidney disease (CKD) and periodontitis, and to explore the relationship between NETs and both diseases.

Methods: 63 CKD and 40 non-CKD participants were recruited and underwent periodontal examination, among which 35 early CKD patients underwent periodontal therapy. The concentrations of NETs were determined by dsDNA assay in gingival crevicular fluid (GCF) and plasma, and by flow cytometry or immunofluorescence assay in blood and gingival tissues. The correlations between NETs and clinical parameters were analyzed. The influence of periodontal therapy on periodontitis, CKD and NETs concentrations was also evaluated.

Results: CKD patients had higher concentrations of NETs in plasma than non-CKD patients, and NETs concentrations were also increased in both GCF and plasma of patients with periodontitis than that of periodontally healthy patients. NETs concentrations were positively correlated with increased clinical parameters of CKD and periodontitis. The positive correlation between CKD and periodontitis was demonstrated. Moreover, periodontal therapy ameliorated periodontitis and CKD, and reduced NETs concentrations in GCF of patients.

Conclusions: This study revealed that NETs might be a possible bridge between periodontitis and CKD, and suggested the potential target for therapy.

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中性粒细胞胞外捕获器在牙周炎与慢性肾脏病之间的交叉作用。

背景：目的：评估慢性肾脏病（CKD）患者和牙周炎患者体内中性粒细胞胞外捕获物（NETs）的水平，并探讨NETs与这两种疾病之间的关系。方法：招募63名CKD患者和40名非CKD患者进行牙周检查，其中35名早期CKD患者接受了牙周治疗。通过dsDNA检测法测定牙龈缝隙液（GCF）和血浆中的NETs浓度，通过流式细胞术或免疫荧光检测法测定血液和牙龈组织中的NETs浓度。分析了NETs与临床参数之间的相关性。此外，还评估了牙周治疗对牙周炎、CKD 和 NETs 浓度的影响：结果：CKD 患者血浆中的 NETs 浓度高于非 CKD 患者，牙周炎患者 GCF 和血浆中的 NETs 浓度也高于牙周健康患者。NETs浓度与CKD和牙周炎临床指标的增加呈正相关。这表明慢性肾功能衰竭与牙周炎之间存在正相关。此外，牙周治疗可改善牙周炎和 CKD，并降低患者 GCF 中的 NETs 浓度：这项研究揭示了 NETs 可能是牙周炎和 CKD 之间的桥梁，并提出了潜在的治疗目标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Oral Health DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.90

自引率

6.90%

发文量

481

审稿时长

6-12 weeks

期刊介绍： BMC Oral Health is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of disorders of the mouth, teeth and gums, as well as related molecular genetics, pathophysiology, and epidemiology.