VascuFit: Aerobic exercise improves endothelial function independent of cardiovascular risk: A randomized-controlled trial

IF 4.9 2区医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS

Atherosclerosis Pub Date : 2024-10-16 DOI:10.1016/j.atherosclerosis.2024.118631

Daniel Goeder , Julia Maria Kröpfl , Thomas Angst , Henner Hanssen , Christoph Hauser , Denis Infanger , Debbie Maurer , Renate Oberhoffer-Fritz , Arno Schmidt-Trucksäss , Karsten Königstein

{"title":"VascuFit: Aerobic exercise improves endothelial function independent of cardiovascular risk: A randomized-controlled trial","authors":"Daniel Goeder , Julia Maria Kröpfl , Thomas Angst , Henner Hanssen , Christoph Hauser , Denis Infanger , Debbie Maurer , Renate Oberhoffer-Fritz , Arno Schmidt-Trucksäss , Karsten Königstein","doi":"10.1016/j.atherosclerosis.2024.118631","DOIUrl":null,"url":null,"abstract":"<div><h3>Background and aims</h3><div>Endothelial dysfunction predicts elevated cardiovascular (CV) risk in healthy individuals. Aerobic exercise reduces endothelial dysfunction in part by improving CV risk factors. Yet, this explains less than 50 % of the effect and a direct influence of exercise training on the endothelium is discussed as possible contributor. The VascuFit study applied non-linear periodized aerobic exercise (NLPE) training to assess its multilevel effects on endothelial function including potential epigenetic endothelial modifications by circulating micro-ribonucleic acids (endomiRs).</div></div><div><h3>Methods</h3><div>Sedentary adults with elevated CV risk between 40 and 60 years were randomized 2:1 and engaged in an eight-week ergometer-based NLPE training (n = 30) or received standard exercise recommendations (n = 14). Macro-, microvascular, cellular and molecular adaptations were assessed via brachial-arterial flow-mediated dilation (baFMD), static retinal vessel analysis (SVA), flow cytometry, and endomiRs regulating key pathways of endothelial function. Statistics included ANCOVA, Principal Component Analysis (PCA), and regression analyses.</div></div><div><h3>Results</h3><div>baFMD improved by 2.38 % (CI:0.70–4.06, <em>p</em> = 0.007) independent of CV risk, whereas SVA parameters and circulating endothelial (progenitor) cells did not significantly change in the NLPE group. The mean distance between baseline and follow-up PCA loadings of the endomiR dataset explaining 44.2 % of dataset variability was higher in the NLPE-group compared to the control group (2.71 ± 2.02 <em>vs.</em> 1.65 ± 0.93). However, regression analyses showed no evidence of endomiRs explaining the improvement of baFMD.</div></div><div><h3>Conclusions</h3><div>The improvement of macrovascular endothelial function by aerobic exercise training was independent from CV risk factors. Increased heterogeneity among endomiRs did not explain this effect, but suggests an adaptive response to the exercise stimulus on the epigenetic level.</div></div>","PeriodicalId":8623,"journal":{"name":"Atherosclerosis","volume":"399 ","pages":"Article 118631"},"PeriodicalIF":4.9000,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Atherosclerosis","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0021915024012036","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CARDIAC & CARDIOVASCULAR SYSTEMS","Score":null,"Total":0}

引用次数: 0

Abstract

Background and aims

Endothelial dysfunction predicts elevated cardiovascular (CV) risk in healthy individuals. Aerobic exercise reduces endothelial dysfunction in part by improving CV risk factors. Yet, this explains less than 50 % of the effect and a direct influence of exercise training on the endothelium is discussed as possible contributor. The VascuFit study applied non-linear periodized aerobic exercise (NLPE) training to assess its multilevel effects on endothelial function including potential epigenetic endothelial modifications by circulating micro-ribonucleic acids (endomiRs).

Methods

Sedentary adults with elevated CV risk between 40 and 60 years were randomized 2:1 and engaged in an eight-week ergometer-based NLPE training (n = 30) or received standard exercise recommendations (n = 14). Macro-, microvascular, cellular and molecular adaptations were assessed via brachial-arterial flow-mediated dilation (baFMD), static retinal vessel analysis (SVA), flow cytometry, and endomiRs regulating key pathways of endothelial function. Statistics included ANCOVA, Principal Component Analysis (PCA), and regression analyses.

Results

baFMD improved by 2.38 % (CI:0.70–4.06, p = 0.007) independent of CV risk, whereas SVA parameters and circulating endothelial (progenitor) cells did not significantly change in the NLPE group. The mean distance between baseline and follow-up PCA loadings of the endomiR dataset explaining 44.2 % of dataset variability was higher in the NLPE-group compared to the control group (2.71 ± 2.02 vs. 1.65 ± 0.93). However, regression analyses showed no evidence of endomiRs explaining the improvement of baFMD.

Conclusions

The improvement of macrovascular endothelial function by aerobic exercise training was independent from CV risk factors. Increased heterogeneity among endomiRs did not explain this effect, but suggests an adaptive response to the exercise stimulus on the epigenetic level.

Abstract Image

查看原文本刊更多论文

VascuFit：有氧运动可改善内皮功能，与心血管风险无关：随机对照试验

背景和目的：内皮功能障碍预示着健康人的心血管（CV）风险升高。有氧运动可通过改善心血管疾病风险因素来减轻内皮功能障碍。然而，这只能解释不到 50% 的效果，运动训练对内皮的直接影响被认为是可能的原因。VascuFit 研究采用非线性周期性有氧运动（NLPE）训练来评估其对内皮功能的多层次影响，包括循环微核糖核酸（endomiRs）对内皮的潜在表观遗传修饰：方法：将年龄在 40 至 60 岁之间、心血管疾病风险较高的久坐成年人按 2:1 随机分组，参加为期八周、以测力计为基础的 NLPE 训练（30 人）或接受标准运动建议（14 人）。通过肱动脉血流介导的扩张（baFMD）、静态视网膜血管分析（SVA）、流式细胞术和调节内皮功能关键通路的内切酶Rs评估宏观、微观血管、细胞和分子适应性。统计包括方差分析、主成分分析（PCA）和回归分析。结果：baFMD改善了2.38%（CI:0.70-4.06，P = 0.007），与CV风险无关，而SVA参数和循环内皮（祖细胞）细胞在NLPE组中没有显著变化。与对照组相比，NLPE 组基线与随访内皮细胞数据集 PCA 负载之间的平均距离更高（2.71 ± 2.02 vs. 1.65 ± 0.93），而 PCA 负载可解释数据集变异性的 44.2%。然而，回归分析表明，没有证据表明内皮生长因子能解释 baFMD 的改善：结论：有氧运动训练对大血管内皮功能的改善与冠心病风险因素无关。结论：有氧运动训练对大血管内皮功能的改善与冠状动脉风险因素无关。内皮生长因子之间的异质性增加并不能解释这种效果，但表明在表观遗传学水平上对运动刺激的适应性反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Atherosclerosis 医学-外周血管病

CiteScore

9.80

自引率

3.80%

发文量

1269

审稿时长

36 days

期刊介绍： Atherosclerosis has an open access mirror journal Atherosclerosis: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Atherosclerosis brings together, from all sources, papers concerned with investigation on atherosclerosis, its risk factors and clinical manifestations. Atherosclerosis covers basic and translational, clinical and population research approaches to arterial and vascular biology and disease, as well as their risk factors including: disturbances of lipid and lipoprotein metabolism, diabetes and hypertension, thrombosis, and inflammation. The Editors are interested in original or review papers dealing with the pathogenesis, environmental, genetic and epigenetic basis, diagnosis or treatment of atherosclerosis and related diseases as well as their risk factors.