Isolation, identification, and challenges of extracellular vesicles: emerging players in clinical applications.

IF 6.1 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Apoptosis Pub Date : 2024-11-10 DOI:10.1007/s10495-024-02036-2

Xiaoxiao Ma, Lanwei Peng, Xiaohui Zhu, Tianqi Chu, Changcheng Yang, Bohao Zhou, Xiangwei Sun, Tianya Gao, Mengqi Zhang, Ping Chen, Haiyan Chen

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引用次数: 0

Abstract

Extracellular vesicles (EVs) serve as critical mediators of intercellular communication, encompassing exosomes, microvesicles, and apoptotic vesicles that play significant roles in diverse physiological and pathological contexts. Numerous studies have demonstrated that EVs derived from mesenchymal stem cells (MSC-EVs) play a pivotal role in facilitating tissue and organ repair, alleviating inflammation and apoptosis, enhancing the proliferation of endogenous stem cells within tissues and organs, and modulating immune function-these functions have been extensively utilized in clinical applications. The precise classification, isolation, and identification of MSC-EVs are essential for their clinical applications. This article provides a comprehensive overview of the biological properties of EVs, emphasizing both their advantages and limitations in isolation and identification methodologies. Additionally, we summarize the protein markers associated with MSC-EVs, emphasizing their significance in the treatment of various diseases. Finally, this article addresses the current challenges and dilemmas in developing clinical applications for MSC-EVs, aiming to offer valuable insights for future research.

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细胞外囊泡的分离、鉴定和挑战：临床应用中的新兴角色。

细胞外囊泡（EVs）是细胞间通讯的关键媒介，包括外泌体、微囊泡和凋亡囊泡，它们在不同的生理和病理环境中发挥着重要作用。大量研究表明，间充质干细胞衍生的EVs（间充质干细胞-EVs）在促进组织和器官修复、减轻炎症和细胞凋亡、增强组织和器官内源性干细胞增殖以及调节免疫功能等方面发挥着关键作用，这些功能已被广泛应用于临床。间充质干细胞的精确分类、分离和鉴定对其临床应用至关重要。本文全面概述了EVs的生物学特性，强调了其在分离和鉴定方法上的优势和局限性。此外，我们还总结了与间充质干细胞-EVs 相关的蛋白质标记物，强调了它们在治疗各种疾病中的意义。最后，本文探讨了目前在开发间充质干细胞-EVs临床应用方面所面临的挑战和困境，旨在为未来的研究提供有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Apoptosis 生物-生化与分子生物学

CiteScore

9.10

自引率

4.20%

发文量

审稿时长

1 months

期刊介绍： Apoptosis, a monthly international peer-reviewed journal, focuses on the rapid publication of innovative investigations into programmed cell death. The journal aims to stimulate research on the mechanisms and role of apoptosis in various human diseases, such as cancer, autoimmune disease, viral infection, AIDS, cardiovascular disease, neurodegenerative disorders, osteoporosis, and aging. The Editor-In-Chief acknowledges the importance of advancing clinical therapies for apoptosis-related diseases. Apoptosis considers Original Articles, Reviews, Short Communications, Letters to the Editor, and Book Reviews for publication.