Limitations in LC-MS/MS application for adagrasib and pembrolizumab quantification in rat plasma

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B Pub Date : 2024-11-01 DOI:10.1016/j.jchromb.2024.124354

Zvonimir Mlinarić

引用次数: 0

Abstract

An article by Harsha Shi et al. raises multiple concerns regarding sample preparation, the presence of the analyte in the analyzed solution, authenticity of the MS spectra, administration of drugs and blood collection, choice of internal standard, obtained pharmacokinetic parameters and usage of chromatographic column and solvents. While the research topic is interesting and the development of bioanalytical methods for novel drugs is crucial, this article does not seem to meet the analytical and methodological standards for the reliable determination of adagrasib and pembrolizumab in plasma samples. A detailed explanation is given in the letter.

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将 LC-MS/MS 应用于大鼠血浆中 adagrasib 和 pembrolizumab 定量的局限性。

Harsha Shi 等人的一篇文章提出了样品制备、分析溶液中分析物的存在、质谱的真实性、给药和采血、内标的选择、获得的药代动力学参数以及色谱柱和溶剂的使用等多方面的问题。虽然研究课题很有意义，开发新型药物的生物分析方法也很关键，但这篇文章似乎不符合可靠测定血浆样本中阿达拉昔布和彭博利珠单抗的分析和方法学标准。信中给出了详细解释。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Chromatography B 医学-分析化学

CiteScore

5.60

自引率

3.30%

发文量

306

审稿时长

44 days

期刊介绍： The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis. Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches. Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.